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PMID: 4923076 Published · ppublish English Journal Article

Genetic and molecular characteristics of X-ray-sensitive mutants of Escherichia coli defective in repair synthesis.

Journal of bacteriology ·Vol. 104 ·No. 2 ·1970-11-00 ·Pages 871-81

Kato T, Kondo S

Abstract

Alleles responsible for X-ray-sensitive characteristics of three mutants of Escherichia coli B, which were also sensitive to ultraviolet (UV) irradiation, were mapped near metE locus, and named res-1, res-2, and res-3. All the res(-) mutants showed no host cell reactivability (Hcr(-)) for transducing deoxyribonucleic acid (DNA) of P1 phage irradiated by UV but they were Hcr(+) for infective DNA of P1 phage. Furthermore, they showed no detectable activity of DNA polymerase. Characteristics of allele res-1 were studied in detail. The mutant res-1 uvr(+) showed an extensive degradation of DNA after UV irradiation. Double mutants carrying res-1 uvrA(-), res-1 uvrB(-), and res-1 uvrC(-) showed no marked increase in UV sensitivity beyond that of the uvr(-) single mutants and only negligible UV-induced DNA degradation. The uvr(-) mutations showed no such suppressive effect on DNA degradation induced by X rays in these double mutants. It is concluded that res(-) mutants are defective in the second step (repair synthesis) of the excision repair process and that DNA polymerase is partly responsible for the assumed resynthesis step.

MeSH Terms
Alleles Centrifugation, Density Gradient Chromosome Mapping Coliphages/radiation effects Crosses, Genetic DNA Nucleotidyltransferases/metabolism DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/enzymology,radiation effects Genes Genetics, Microbial Mutation Nucleotides/metabolism Radiation Effects Sucrose Transduction, Genetic Tritium Ultraviolet Rays
Chemicals
DNA, Bacterial Nucleotides Tritium Sucrose DNA Nucleotidyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kato T
Kondo S
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.104.2.871-881.1970
Published
1970-11-00
Pages
871-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC285071
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.104.2.871-881.1970 PMC Full Text View journal details
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