Abstract
Further evidence is presented to confirm the previous conclusion that the enzyme from Escherichia coli catalysing the two sequential reactions in tryptophan biosynthesis, N-(5'-phosphoribosyl)anthranilic acid (PRA) --> 1-(o-carboxyphenyl-amino)-1-deoxyribulose 5-phosphate (CdRP) --> indol-3-ylglycerol phosphate (InGP)+CO(2)+H(2)O, consists of a single polypeptide chain. The kinetic properties of the enzyme demonstrate that intermediate CdRP formed from PRA must dissociate from the enzyme before it can be converted into InGP. It is concluded that there are two distinct and non-overlapping catalytic sites on the enzyme for the two reactions. The expected complementation between a mutationally altered form of the enzyme lacking the first reaction and a mutationally altered form lacking the second reaction has been demonstrated in vitro by InGP formation from PRA. This system thus exhibits intracistronic complementation with a non-oligomeric protein gene product.
MeSH Terms
Amino Sugars/biosynthesis,metabolism
Binding Sites
Carboxy-Lyases/metabolism
Chromatography, Gel
Coliphages
Electrophoresis
Escherichia coli/enzymology
Genetic Complementation Test
Genetics, Microbial
Glycerophosphates
Indoles/biosynthesis
Isomerases/analysis,metabolism
Kinetics
Molecular Weight
Mutation
Pentosephosphates/biosynthesis,metabolism
Peptides/analysis
Phosphotransferases/analysis
Tryptophan/biosynthesis
ortho-Aminobenzoates/biosynthesis,metabolism
Chemicals
Amino Sugars
Glycerophosphates
Indoles
Pentosephosphates
Peptides
ortho-Aminobenzoates
Tryptophan
Phosphotransferases
Carboxy-Lyases
Isomerases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Creighton T E
References (26)
26 references, click to expand
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