Home LiteratureArticle Details
PMID: 4924970 Published · ppublish English Journal Article

Chemical modifications of transfer RNA species. Desulfurization with Raney nickel.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 1 ·1971-01-00 ·Pages 48-51

Hecht SM, Kirkegaard LH, Bock RM

Abstract

A single, modified nucleoside in Escherichia coli tRNA, 6-(3-methyl-2-butenylamino)-2-methyl-thio-9-beta-D-ribofuranosylpurine, has been desulfurized with Raney nickel to afford its probable biosynthetic precursor. The limitations of the reaction at the nucleoside and tRNA levels and its lack of inhibition of the amino acid acceptor activity of tRNA are described.

MeSH Terms
Catalysis Chemical Phenomena Chemistry Chromatography, Paper Escherichia coli/metabolism Nickel Nucleosides/analysis Phenylalanine/metabolism Plant Growth Regulators RNA, Bacterial/biosynthesis RNA, Transfer/biosynthesis Serine/metabolism Spectrum Analysis Sulfur Tyrosine/metabolism
Chemicals
Nucleosides Plant Growth Regulators RNA, Bacterial Tyrosine Serine Phenylalanine Sulfur Nickel RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hecht S M
Kirkegaard L H
Bock R M
References (20)
20 references, click to expand
  1. Cytokinin from soluble RNA of Escherichia coli: 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine.
    Science. 1968 Aug 16;161(3842):691-3 PMID: 4874577
  2. Cytokinins: distribution in transfer RNA species of Escherichia coli.
    Proc Natl Acad Sci U S A. 1969 Jul;63(3):834-41 PMID: 4899879
  3. 2-Methylthio N6-(delta 2-isopentenyl) adenosine: a component of E. coli tyrosine transfer RNA.
    Biochem Biophys Res Commun. 1968 Oct 24;33(2):299-306 PMID: 4881052
  4. The isolation and identification of two cytokinins from Escherichia coli transfer ribonucleic acids.
    Biochemistry. 1969 Jul;8(7):3071-6 PMID: 4897210
  5. Transfer ribonucleic acids.
    Angew Chem Int Ed Engl. 1969 Oct;8(10):711-27 PMID: 4982498
  6. The incorporation of mevalonic acid into the N6-(delta 2-isopentenyl) adenosine of transfer ribonucleic acid in Lactobacillus acidophilus.
    Biochemistry. 1968 Jan;7(1):472-82 PMID: 4991483
  7. Phenylalanine transfer ribonucleic acid from Escherichia coli B. Isolation and characterization of oligonucleotides from ribonuclease T-1 and ribonuclease A hydrolysates.
    Biochemistry. 1969 Apr;8(4):1643-55 PMID: 4896461
  8. An iron-dependent modification of several transfer RNA species in Escherichia coli.
    J Mol Biol. 1969 Dec 28;46(3):581-4 PMID: 4904109
  9. 6-(gamma,gamma-Dimethylallylamino)Purine As A Precursor of Zeatin.
    Plant Physiol. 1969 Mar;44(3):372-6 PMID: 16657072
  10. ISOLATION OF N6-(AMINOACYL)ADENOSINE FROM YEAST RIBONUCLEIC ACID.
    Biochemistry. 1964 Jun;3:769-73 PMID: 14211613
  11. Amber suppression: a nucleotide change in the anticodon of a tyrosine transfer RNA.
    Nature. 1968 Mar 16;217(5133):1019-24 PMID: 5643523
  12. Cytokinin of wheat germ transfer RNA: 6-(4-hydroxy-3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine.
    Science. 1969 Dec 5;166(3910):1272-4 PMID: 5350318
  13. Biosynthesis of N6-(delta-2-isopentenyl) adenosine. The precursor relationship of acetate and mevalonate to the delta-2-isopentenyl group of the transfer ribonucleic acid of microorganisms.
    Biochemistry. 1968 Mar;7(3):940-4 PMID: 5657858
  14. Cytokinins: synthesis, mass spectra, and biological activity of compounds related to zeatin.
    Proc Natl Acad Sci U S A. 1969 May;63(1):175-82 PMID: 16591745
  15. The presence of 2-methylthio-N6-(delta-2-isopentenyl) adenosine in serine and phenylalanine transfer RNA's from Escherichia coli.
    Biochim Biophys Acta. 1969 Apr 22;179(2):517-20 PMID: 4890605
  16. N6-(Delta 2-isopentenyl)adenosine: biosynthesis in vitro by an enzyme extract from yeast and rat liver.
    Biochem Biophys Res Commun. 1968 May 23;31(4):571-6 PMID: 4297772
  17. Role modifications in tyrosine transfer RNA: a modified base affecting ribosome binding.
    J Mol Biol. 1969 Jan 14;39(1):145-57 PMID: 4938812
  18. Conformation of the anticodon loop intRNA.
    Nature. 1967 Aug 19;215(5103):817-21 PMID: 6049728
  19. Potential antimetabolites. IV. Synthesis of 2,6-bis-alkylthiopurine ribosides and their selective substitution by nucleophilic reagents.
    Chem Pharm Bull (Tokyo). 1962 Aug;10:665-9 PMID: 13956289
  20. N6-(delta-2-isopentenyl) adenosine. Biosynthesis in transfer ribonucleic acid in vitro.
    Biochemistry. 1969 Nov;8(11):4361-71 PMID: 4311031
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.1.48
Published
1971-01-00
Pages
48-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391099
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.1.48 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2