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PMID: 4926684 Published · ppublish English Journal Article

Genetic analysis of diaminopimelic acid- and lysine-requiring mutants of Escherichia coli.

Journal of bacteriology ·Vol. 105 ·No. 3 ·1971-03-00 ·Pages 844-54

Bukhari AI, Taylor AL

Abstract

Several diaminopimelic acid (DAP)- and lysine-requiring mutants of Escherichia coli were isolated and studied by genetic, physiological, and biochemical means. The genes concerned with DAP-lysine synthesis map at several different sites on the E. coli chromosome and, therefore, do not constitute a single operon. Three separate loci affecting DAP synthesis are located in the 0 to 2.5 min region of the genetic map. The order of the loci in this region is thr-dapB-pyrA-ara-leu-pan-dapC-tonA-dapD. Two additional DAP genes map in the region between min 47 and 48, with the gene order being gua-dapA-dapE-ctr. The lys locus at min 55 determines the synthesis of the enzyme DAP decarboxylase, which catalyzes the conversion of DAP into lysine. The order of the genes in this region is serA-lysA-thyA.

MeSH Terms
Carboxy-Lyases/biosynthesis,metabolism Cell-Free System Chromatography, Paper Chromosome Mapping Colorimetry Conjugation, Genetic Culture Media Escherichia coli/enzymology,growth & development,isolation & purification,metabolism Genes Genetics, Microbial Isomerases/metabolism Lysine/biosynthesis,metabolism Mutation Pimelic Acids/biosynthesis,metabolism Transduction, Genetic
Chemicals
Culture Media Pimelic Acids Carboxy-Lyases Isomerases Lysine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bukhari A I
Taylor A L
References (32)
32 references, click to expand
  1. Variation of activity of bacterial diaminopimelate decarboxylase under different conditions of growth.
    Biochem J. 1964 Jun;91(3):600-10 PMID: 4953932
  2. [Isolation and properties of a lysine-sensitive mutant of Escherichia coli devoid of aspartokinase].
    Biochim Biophys Acta. 1965 Jun 22;99(3):561-3 PMID: 4378825
  3. The condensation step in diaminopimelate synthesis.
    J Biol Chem. 1965 Dec;240(12):4710-6 PMID: 5321309
  4. The reduction step in diaminopimelic acid biosynthesis.
    J Biol Chem. 1965 Dec;240(12):4717-22 PMID: 4378965
  5. Biochemistry of bacterial cell walls.
    Annu Rev Biochem. 1966;35:457-84 PMID: 5329466
  6. Location of the maltose A and B loci on the genetic map of Escherichia coli.
    J Bacteriol. 1966 Oct;92(4):1083-9 PMID: 5332865
  7. Allosteric regulation of enzyme activity.
    Adv Enzymol Relat Areas Mol Biol. 1966;28:41-154 PMID: 5334065
  8. A proposal for a uniform nomenclature in bacterial genetics.
    Genetics. 1966 Jul;54(1):61-76 PMID: 5961488
  9. Revised linkage map of Escherichia coli.
    Bacteriol Rev. 1967 Dec;31(4):332-53 PMID: 4865540
  10. The relationship of dipicolinate and lysine biosynthesis in Bacillus megaterium.
    J Biol Chem. 1968 Jul 25;243(14):3871-6 PMID: 4969366
  11. Some aspects of amino acid biosynthesis in microorganisms.
    Annu Rev Biochem. 1968;37:79-108 PMID: 4875725
  12. Mutant of Escherichia coli with anomalous cell division and ability to decrease episomally and chromosomally mediated resistance to ampicillin and several other antibiotics.
    J Bacteriol. 1969 Mar;97(3):1334-42 PMID: 4887513
  13. Carbohydrate accumulation and metabolism in Escherichia coli: the close linkage and chromosomal location of ctr mutations.
    J Bacteriol. 1969 May;98(2):605-10 PMID: 4891263
  14. Biochemical characterization of lysine auxotrophs of Staphylococcus aureus.
    J Bacteriol. 1969 Jul;99(1):169-74 PMID: 5802602
  15. Genetic and biochemical studies on cell wall peptidoglycan synthesis in Escherichia coli K-12.
    Biochem Biophys Res Commun. 1969 Aug 15;36(4):682-9 PMID: 4897411
  16. Mapping of the nadB locus adjacent to a previously undescribed purine locus in Escherichia coli K-12.
    J Bacteriol. 1970 May;102(2):377-81 PMID: 4315893
  17. Current linkage map of Escherichia coli.
    Bacteriol Rev. 1970 Jun;34(2):155-75 PMID: 4918631
  18. Mutants of Escherichia coli with a growth requirement for either lysine or pyridoxine.
    J Bacteriol. 1971 Mar;105(3):988-98 PMID: 4926690
  19. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  20. Studies of the bacterial cell wall. IV. The composition of the cell walls of some Gram-positive and Gram-negative bacteria.
    Biochim Biophys Acta. 1953 Apr;10(4):512-23 PMID: 13059016
  21. The stereoisomers of alpha epsilon-diaminopimelic acid. III. Properties and distribution of diaminopimelic acid racemase, an enzyme causing interconversion of the LL and meso isomers.
    Biochem J. 1957 Mar;65(3):448-59 PMID: 13412646
  22. Hybridization between Escherichia coli and Shigella.
    J Bacteriol. 1957 Oct;74(4):461-76 PMID: 13475269
  23. The enzymatic synthesis of diaminopimelic acid.
    J Biol Chem. 1958 Dec;233(6):1501-4 PMID: 13610862
  24. N-Succinyl-alpha-amino-6-ketopimelic acid.
    J Biol Chem. 1961 May;236:1429-31 PMID: 13705452
  25. N-Succinyl-L-diaminopimelic-glutamic transaminase.
    J Biol Chem. 1961 May;236:1432-8 PMID: 13734750
  26. N-Succinyl-L-2,6-diaminopimelic acid deacylase.
    J Biol Chem. 1960 Dec;235:3532-5 PMID: 13756049
  27. The branching point in diaminopimelic acid synthesis.
    J Biol Chem. 1962 Feb;237:482-4 PMID: 13898711
  28. BACTERIOPHAGE-INDUCED MUTATION IN ESCHERICHIA COLI.
    Proc Natl Acad Sci U S A. 1963 Dec;50:1043-51 PMID: 14096176
  29. PURIFICATION AND PROPERTIES OF DIAMINOPIMELATE DECARBOXYLASE FROM ESCHERICHIA COLI.
    Biochem J. 1965 Jul;96:75-84 PMID: 14343156
  30. A new naturally occurring amino acid.
    Nature. 1950 Jan 14;165(4185):74 PMID: 15403106
  31. The stereoisomers of alpha epsilon-diaminopimelic acid: their distribution in nature and behaviour towards certain enzyme preparations.
    Biochem J. 1955 Dec;61(4):562-8 PMID: 13276337
  32. N-Succinyl-L-diaminopimelic acid.
    J Biol Chem. 1959 Nov;234:2955-9 PMID: 13850174
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.105.3.844-854.1971
Published
1971-03-00
Pages
844-54
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248509
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.105.3.844-854.1971 PMC Full Text View journal details
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