Chromosomal integration of F' factors in recombination-deficient Hfr strains of Escherichia coli.

Journal of bacteriology ·Vol. 106 ·No. 1 ·1971-04-00 ·Pages 150-6

DeVries JK, Maas WK

Abstract

In matings between F' donors and recombination-deficient Hfr recipients, we isolated progeny which transferred both episomal markers and Hfr markers early and with high frequency. A number of these progeny had two integrated sex factors. Investigation of these double Hfr strains showed that the F' nearly always integrated in a homologous region of the chromosome. In any particular mating system integration was specific as to location and direction of chromosome transfer.

MeSH Terms

Chromosome Mapping Chromosomes, Bacterial Culture Media DNA, Bacterial Escherichia coli/growth & development,radiation effects Genes Genotype Lactose Mutation Phenotype Recombination, Genetic Streptomycin Ultraviolet Rays

Chemicals

Culture Media DNA, Bacterial Lactose Streptomycin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

DeVries J K

Maas W K

References (8)

8 references, click to expand

A proposal for a uniform nomenclature in bacterial genetics.
Genetics. 1966 Jul;54(1):61-76 PMID: 5961488
Inhibition of replication of an F'lac episome in Hfr cells of Escherichia coli.
J Bacteriol. 1968 Feb;95(2):531-9 PMID: 4867745
Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12.
Proc Natl Acad Sci U S A. 1968 May;60(1):160-7 PMID: 4873517
A mutant of Escherichia coli permitting replication of two F factors.
Proc Natl Acad Sci U S A. 1969 Mar;62(3):873-80 PMID: 4895215
Recombination in bacteriophage lambda. 3. Studies on the nature of the prophage attachment region.
J Mol Biol. 1969 Dec 28;46(3):543-63 PMID: 4904107
Genetic analysis of a "double male" strain of Escherichia coli K-12.
Genetics. 1963 Jan;48:105-20 PMID: 14021492
ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12.
Proc Natl Acad Sci U S A. 1965 Feb;53:451-9 PMID: 14294081
A QUICK AND EFFICIENT METHOD FOR INTERRUPTION OF BACTERIAL CONJUGATION.
Genet Res. 1965 Jul;6:300-3 PMID: 14345913

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.106.1.150-156.1971

Published

1971-04-00

Pages

150-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC248655

Subset

External Links

PubMed source DOI: 10.1128/jb.106.1.150-156.1971 PMC Full Text View journal details

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Chromosomal integration of F' factors in recombination-deficient Hfr strains of Escherichia coli.

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