Home LiteratureArticle Details
PMID: 4930243 Published · ppublish English Journal Article

Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 5 ·1971-05-00 ·Pages 959-63

Senior BW, Holland IB

Abstract

The properties of 30S ribosomal subunits from untreated and from colicin E3-treated (E3-30S) bacteria have been compared. Polyacrylamide gel electrophoresis of ribosomal proteins revealed no difference, but several studies indicated that the 16S RNA from E3-30S particles was modified. E3-16S RNA showed slightly increased resistance to heat-induced degradation and had a reduced (15 S) sedimentation coefficient on sucrose gradients. Fingerprint analyses of E3-16S RNA revealed that the 3'-terminus of the molecule had been deleted. It was concluded that a primary effect of colicin E3 is the activation of a highly specific RNase that degrades 30S ribosomal RNA in situ, and that the resulting fragment(s) are probably retained within the 30S particle.

MeSH Terms
Autoradiography Bacterial Proteins/analysis Centrifugation, Density Gradient Colicins/pharmacology Densitometry Electrophoresis, Disc Escherichia coli/cytology Nucleoproteins/analysis RNA, Ribosomal/metabolism Ribonucleases/metabolism Ribosomes/analysis,drug effects
Chemicals
Bacterial Proteins Colicins Nucleoproteins RNA, Ribosomal Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Senior B W
Holland I B
References (21)
21 references, click to expand
  1. Some observations on the mode of action of colicin F.
    Biochem Biophys Res Commun. 1963 Apr 23;11:140-5 PMID: 13986413
  2. Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo.
    Proc Natl Acad Sci U S A. 1971 May;68(5):964-8 PMID: 4930244
  3. Effects of colicins E1 and K on transport systems.
    J Bacteriol. 1969 Jan;97(1):57-63 PMID: 4884823
  4. Interaction of colicins with bacterial cells. I. Studies with radioactive colicins.
    J Bacteriol. 1966 Feb;91(2):685-94 PMID: 5327363
  5. Mutants of Escherichia coli with altered surface properties which are refractory to colicin E2, sensitive to ultraviolet light and which can also show recombination deficiency, abortive growth of bacteriophage lambda and filament formation.
    J Gen Microbiol. 1970 Aug;62(3):371-82 PMID: 4924623
  6. Envelope protein changes in mutants of Escherichia coli refractory to colicin E2.
    FEBS Lett. 1970 Nov 9;11(1):33-36 PMID: 11945443
  7. MECHANISM OF ACTION OF COLICINES.
    Proc Natl Acad Sci U S A. 1964 Dec;52:1514-21 PMID: 14243526
  8. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.
    Eur J Biochem. 1969 Dec;11(2):395-9 PMID: 4902612
  9. Kinetics of adsorption of colicin CA42-E2 and reversal of its bactericidal activity.
    J Bacteriol. 1969 Oct;100(1):301-9 PMID: 4898995
  10. Nucleotide sequences from the low molecular weight ribosomal RNA of Escherichia coli.
    J Mol Biol. 1967 Feb 14;23(3):337-53 PMID: 4291728
  11. The kinetics of ribonucleic acid- and protein formation in Salmonella typhimurium during the transition between different states of balance growth.
    Biochim Biophys Acta. 1961 Apr 29;49:64-76 PMID: 13756517
  12. A two-dimensional fractionation procedure for radioactive nucleotides.
    J Mol Biol. 1965 Sep;13(2):373-98 PMID: 5325727
  13. Colicin E3-directed changes in ribosome function and polyribosome metabolism in Escherichia coli K12.
    J Mol Biol. 1970 Oct 28;53(2):205-20 PMID: 4923998
  14. Use of Brij lysis as a general method to prepare polyribosomes from Escherichia coli.
    Biochim Biophys Acta. 1967 Dec 19;149(2):489-95 PMID: 4866435
  15. Interaction of colicins with bacterial cells. II. Specific alteration of Escherichia coli ribosomes induced by colicin E3 in vivo.
    J Mol Biol. 1967 Jun 14;26(2):181-95 PMID: 5340611
  16. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.
    Biochemistry. 1969 Jul;8(7):2897-905 PMID: 4897206
  17. Nucleotide sequences present within the 16S ribosomal RNA of Escherichia coli.
    Nature. 1970 Jan 3;225(5227):26-9 PMID: 4903099
  18. Action of T1 ribonuclease on Escherichia coli ribosomes and their subunits.
    Eur J Biochem. 1970 Apr;13(3):577-82 PMID: 4910390
  19. Colicins and related bacteriocins.
    Annu Rev Microbiol. 1967;21:257-84 PMID: 4860260
  20. Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B".
    Proc Natl Acad Sci U S A. 1946 May;32(5):120-8 PMID: 16588724
  21. Reversibility of inhibition of nucleic acids and protein synthesis by colicin K.
    Biochem Biophys Res Commun. 1962 May 4;7:306-9 PMID: 14480262
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.5.959
Published
1971-05-00
Pages
959-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389090
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.5.959 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2