Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.

Applied microbiology ·Vol. 21 ·No. 4 ·1971-04-00 ·Pages 585-7

Lachica RV, Genigeorgis C, Hoeprich PD

Abstract

Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations as low as 0.005 mug/ml in agar and broth cultures. The interactions of agar and deoxyribonucleic acid with Toluidine Blue O are discussed.

MeSH Terms

Agar Bacteriological Techniques Coloring Agents DNA Deoxyribonucleases/metabolism Hydrolysis Methods Micrococcus/enzymology Staphylococcus/enzymology

Chemicals

Coloring Agents Agar DNA Deoxyribonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Lachica R V

Genigeorgis C

Hoeprich P D

References (5)

5 references, click to expand

Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus.
J Biol Chem. 1967 Apr 10;242(7):1541-7 PMID: 4290246
Relationships among coagulase, enterotoxin, and heat-stable deoxyribonuclease production by Staphylococcus aureus.
Appl Microbiol. 1969 Jul;18(1):126-7 PMID: 4896099
Detection of nuclease activity in semisolid and broth cultures.
Appl Microbiol. 1969 Aug;18(2):174-6 PMID: 4185235
Evaluation of extracellular deoxyribonuclease activity in Pseudomonas.
J Bacteriol. 1962 Jul;84:77-80 PMID: 13917754
The structure of the DNA-acridine complex.
Proc Natl Acad Sci U S A. 1963 Jan 15;49:94-102 PMID: 13929834

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.21.4.585-587.1971

Published

1971-04-00

Pages

585-7

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC377235

Subset

External Links

PubMed source DOI: 10.1128/am.21.4.585-587.1971 PMC Full Text

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