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PMID: 4934061 Published · ppublish English Journal Article

Promoter-like mutant with increased expression of the glycerol kinase operon of Escherichia coli.

Journal of bacteriology ·Vol. 106 ·No. 3 ·1971-06-00 ·Pages 724-31

Berman-Kurtz M, Lin EC, Richey DP

Abstract

A glycerol-specific phenotypic revertant isolated from a mutant of Escherichia coli missing enzyme I of the phosphoenolpyruvate phosphotransferase system was studied. This revertant is capable of producing higher levels of glycerol kinase and the protein mediating the facilitated diffusion of glycerol (facilitator) than wild-type cells. The kinase of the revertant is indistinguishable from the wild-type enzyme with respect to its sensitivity to feedback inhibition by fructose-1,6-diphosphate, its pH optimum, and its turnover number. The synthesis of glycerol kinase in strains bearing the suppressor locus is resistant to catabolite repression. The suppressor mutation mapped at the known glpK locus. Thus, it is suggested that the mutation occurred in the promoter of the operon specifying the kinase and the facilitator.

MeSH Terms
Arabinose/metabolism Carbon Isotopes Chromosome Mapping Coliphages Culture Media Enzyme Induction Enzyme Repression Escherichia coli/enzymology,growth & development,immunology Genetics, Microbial Glycerol/metabolism Glycerolphosphate Dehydrogenase/metabolism Glycerophosphates/metabolism Hydrogen-Ion Concentration Immune Sera Mutation Phenotype Phosphotransferases/biosynthesis,metabolism Precipitin Tests Suppression, Genetic Transduction, Genetic
Chemicals
Carbon Isotopes Culture Media Glycerophosphates Immune Sera Arabinose Glycerolphosphate Dehydrogenase Phosphotransferases Glycerol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Berman-Kurtz M
Lin E C
Richey D P
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36 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.106.3.724-731.1971
Published
1971-06-00
Pages
724-31
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248685
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.106.3.724-731.1971 PMC Full Text View journal details
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