Kinetics of exogenous induction of the hexose-6-phosphate transport system of Escherichia coli.

Journal of bacteriology ·Vol. 107 ·No. 1 ·1971-07-00 ·Pages 74-8

Winkler HH

Abstract

The kinetics of the exogenous induction of the hexose-phosphate transport system by glucose-6-phosphate (G6P) was investigated. The induction of this system by extracellular but not intracellular G6P was confirmed. The differential rate of synthesis was linear, a function of the extracellular concentration of G6P and independent of the previous induction history of the culture. Neither maintenance nor autocatalysis, phenomena described in the induction of the lac operon, were observed in the exogenous induction of hexose-phosphate transport. Fructose-6-phosphate, a potent competitive inhibitor of G6P influx, had no effect on the induction of the system by G6P, indicating that the transport of inducer was not involved in the induction process.

MeSH Terms

Biological Transport, Active Carbon Isotopes Chloramphenicol Culture Media Enzyme Induction Enzyme Repression Escherichia coli/enzymology,growth & development,metabolism Filtration Fructose/metabolism,pharmacology Galactosidases/biosynthesis Genetics, Microbial Glucose/metabolism,pharmacology Glucose-6-Phosphate Isomerase/biosynthesis Hexosephosphates/metabolism,pharmacology Spectrophotometry

Chemicals

Carbon Isotopes Culture Media Hexosephosphates Fructose Chloramphenicol Galactosidases Glucose-6-Phosphate Isomerase Glucose

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Winkler H H

References (10)

10 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.107.1.74-78.1971

Published

1971-07-00

Pages

74-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC246887

Subset

External Links

PubMed source DOI: 10.1128/jb.107.1.74-78.1971 PMC Full Text View journal details

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Kinetics of exogenous induction of the hexose-6-phosphate transport system of Escherichia coli.

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