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PMID: 4935336 Published · ppublish English Journal Article

A fine structural study of adhesive cell junctions in heterotypic cell aggregates.

The Journal of cell biology ·Vol. 47 ·No. 1 ·1970-10-00 ·Pages 197-210

Armstrong PB

Abstract

Mixed suspensions of cells obtained by dissociation of 7 day chicken embryo heart and pigmented retina were allowed to reaggregate in tissue culture. The reaggregates which resulted contained both kinds of cells. Establishment of homogeneous tissues by cell sorting out in these reaggregates was advanced by 20 hr in culture and was complete within 2 days. When sorting out was advanced, heterotypic aggregates were fixed, sectioned, and examined in the electron microscope. Particular attention was paid to the morphology of regions of contact between cells. No qualitative differences were observed in the contact junctions between like cells (heart-heart or pigmented retina-pigment retina junctions) and unlike cells (heart-pigmented retina junctions). Broad areas of undifferentiated cell contact with cell membranes separated by a 100-200 A gap were formed regardless of cell type. Specialized junctions of the fascia and macula adherens type were also present, not only between like cells but also between unlike cells.

MeSH Terms
Animals Cell Adhesion Cell Aggregation Cell Fractionation Cell Membrane Chick Embryo Culture Techniques Heart Ventricles/embryology Intercellular Junctions Microscopy, Electron Microscopy, Phase-Contrast Pseudopodia Retina/embryology Time Factors
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Armstrong P B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.47.1.197
Published
1970-10-00
Pages
197-210
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108387
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.47.1.197 PMC Full Text View journal details
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