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PMID: 49354 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.

The Journal of cell biology ·Vol. 66 ·No. 1 ·1975-07-00 ·Pages 188-93

Krishan A

Abstract

A rapid method for the flow microfluorometric determination of the DNA content per cell is described. Incubation of cells in a hypotonic solution of propidium iodide results in disruption of the cell membrane and rapid staining of nuclear chromatin. DNA distribution histograms generated from cells stained by this method are identical to those generated after fixation and RNase digestion. In contrast to some earlier described methods, the present technique is rapid (5 min of processing), requires a minimal amount of material, and avoids formation of cell clumps.

MeSH Terms
Bone Marrow/analysis Bone Marrow Cells Cell Division Cell Line DNA/analysis DNA, Neoplasm/analysis Erythrocytes/analysis Ethidium/analogs & derivatives Evaluation Studies as Topic Fluorometry/standards Humans Leukemia, Lymphoid Methanol Ribonucleases/metabolism Staining and Labeling/methods
Chemicals
DNA, Neoplasm DNA Ribonucleases Ethidium Methanol
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Krishan A
References (8)
8 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.66.1.188
Published
1975-07-00
Pages
188-93
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109516
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.66.1.188 PMC Full Text View journal details
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