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PMID: 4939059 Published · ppublish English Journal Article

Properties of bacteriophage T4 mutants defective in gene 30 (deoxyribonucleic acid ligase) and the rII gene.

Journal of virology ·Vol. 7 ·No. 2 ·1971-02-00 ·Pages 260-6

Karam JD, Barker B

Abstract

In Escherichia coli K-12 strains infected with phage T4 which is defective in gene 30 [deoxyribonucleic acid (DNA) ligase] and in the rII gene (product unknown), near normal levels of DNA and viable phage were produced. Growth of such T4 ligase-rII double mutants was less efficient in E. coli B strains which show the "rapidlysis" phenotype of rII mutations. In pulse-chase experiments coupled with temperature shifts and with inhibition of DNA synthesis, it was observed that DNA synthesized by gene 30-defective phage is more susceptible to breakdown in vivo when the phage is carrying a wild-type rII gene. Breakdown was delayed or inhibited by continued DNA synthesis. Mutations of the rII gene decreased but did not completely abolish the breakdown. T4 ligase-rII double mutants had normal sensitivity to ultraviolet irradiation.

MeSH Terms
Coliphages/enzymology,growth & development,metabolism,radiation effects Culture Media DNA Nucleotidyltransferases/biosynthesis DNA Repair DNA Replication DNA, Bacterial/biosynthesis DNA, Viral/biosynthesis Escherichia coli/growth & development,metabolism Genes Genetics, Microbial Molecular Biology Mutation Radiation Effects Thymidine/metabolism Tritium Ultraviolet Rays Virus Replication
Chemicals
Culture Media DNA, Bacterial DNA, Viral Tritium DNA Nucleotidyltransferases Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Karam J D
Barker B
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31 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.7.2.260-266.1971
Published
1971-02-00
Pages
260-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356107
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.7.2.260-266.1971 PMC Full Text View journal details
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