Home LiteratureArticle Details
PMID: 4939759 Published · ppublish English Journal Article

Growth rate of Escherichia coli at elevated temperatures: reversible inhibition of homoserine trans-succinylase.

Journal of bacteriology ·Vol. 107 ·No. 2 ·1971-08-00 ·Pages 397-400

Ron EZ, Shani M

Abstract

The preceding paper (10) showed that the growth of Escherichia coli is slowed, without killing, at 40 to 45 C, and that in the several strains tested the cause is a decrease in the activity of homoserine trans-succinylase. These temperatures are now shown to inhibit the enzyme directly, in crude extracts and after partial purification. The effect is rapid and is immediately reversible, unlike the progressive and slowly reversible changes of conventional denaturation.

MeSH Terms
Acyltransferases/metabolism Amino Acids/biosynthesis Ammonium Sulfate Bacterial Proteins/biosynthesis Carbon Isotopes Cell Fractionation Cell-Free System Chemical Precipitation Coenzyme A/metabolism Culture Media Escherichia coli/enzymology,growth & development,metabolism Homoserine/metabolism Hot Temperature Leucine/metabolism Ligases/antagonists & inhibitors,isolation & purification Mercaptoethanol/pharmacology Methionine/metabolism Protein Denaturation Spectrophotometry Succinates/metabolism Urea/pharmacology
Chemicals
Amino Acids Bacterial Proteins Carbon Isotopes Culture Media Succinates Mercaptoethanol Homoserine Urea Methionine Acyltransferases Ligases Leucine Coenzyme A Ammonium Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ron E Z
Shani M
References (10)
10 references, click to expand
  1. Mutants of Escherichia coli requiring methionine or vitamin B12.
    J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
  2. Protein denaturation.
    Adv Protein Chem. 1968;23:121-282 PMID: 4882248
  3. A BASIC MECHANISM IN THE BIOLOGICAL EFFECTS OF TEMPERATURE, PRESSURE AND NARCOTICS.
    Science. 1942 Feb 20;95(2460):200-3 PMID: 17745078
  4. Multimetabolite control of a biosynthetic pathway by sequential metabolites.
    J Biol Chem. 1966 Nov 25;241(22):5479-80 PMID: 5333667
  5. Allosteric proteins and cellular control systems.
    J Mol Biol. 1963 Apr;6:306-29 PMID: 13936070
  6. Mutants of Escherichia coli with High Minimal Temperatures of Growth.
    J Bacteriol. 1965 Sep;90(3):611-6 PMID: 16562056
  7. Tissue sulfhydryl groups.
    Arch Biochem Biophys. 1959 May;82(1):70-7 PMID: 13650640
  8. Growth rate of Escherichia coli at elevated temperatures: limitation by methionine.
    J Bacteriol. 1971 Aug;107(2):391-6 PMID: 4939758
  9. Inhibition of growth of Escherichia coli and of homoserine O-transsuccinylase by alpha-methylmethionine.
    J Bacteriol. 1967 Aug;94(2):327-32 PMID: 5341861
  10. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.107.2.397-400.1971
Published
1971-08-00
Pages
397-400
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246938
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.107.2.397-400.1971 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2