Altered ribosomes after inhibition of Escherichia coli by rifampicin.

The Biochemical journal ·Vol. 121 ·No. 3 ·1971-02-00 ·Pages 391-8

Blundell MR, Wild DG

Abstract

Addition of rifampicin to growing cells of Escherichia coli affected the ribosomes. The polyribosomes first decayed to 70S ribosomes. These later dissociated to particles distinct from ribosomal subunits. The altered ribosomes sedimented more slowly than the corresponding subunits and had lost some protein; their ribosomal RNA was intact, but they were more susceptible to degradation by ribonuclease than normal ribosomes. The addition of rifampicin to preparations of lysed cells caused no detectable changes in the ribosome fraction.

MeSH Terms

Carbon Isotopes Cell Division/drug effects Centrifugation, Density Gradient Escherichia coli/drug effects Lysine/metabolism Phosphorus Isotopes RNA, Bacterial/analysis RNA, Ribosomal Ribonucleases/metabolism Ribosomes/drug effects Rifampin/pharmacology Tritium Uracil/metabolism

Chemicals

Carbon Isotopes Phosphorus Isotopes RNA, Bacterial RNA, Ribosomal Tritium Uracil Ribonucleases Lysine Rifampin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Blundell M R

Wild D G

References (12)

12 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1210391

Published

1971-02-00

Pages

391-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1176585

Subset

External Links

PubMed source DOI: 10.1042/bj1210391 PMC Full Text View journal details

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Altered ribosomes after inhibition of Escherichia coli by rifampicin.

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