Polysome turnover during amino acid starvation in Escherichia coli.

Journal of bacteriology ·Vol. 108 ·No. 1 ·1971-10-00 ·Pages 263-8

Ron EZ

Abstract

The experiments presented in this paper support earlier evidence that ribosomes are released from polysomes when they encounter a codon for which no charged transfer ribonucleic acid is available. However, it is further shown that these ribosomes then reinitiate and resume translation. The size and the level of polysomes during deprival of an amino acid is a function of the frequency with which that particular amino acid appears in cellular proteins. Polysomes from starved cells are more stable than those from growing cells, and, moreover, polysomes from starved relaxed strains are more stable than those from starved stringent strains.

MeSH Terms

Amino Acids/metabolism Bacterial Proteins/biosynthesis Carbon Isotopes Centrifugation, Density Gradient Culture Media Escherichia coli/growth & development,metabolism Genetics, Microbial Mutation Peptide Biosynthesis RNA, Bacterial/biosynthesis RNA, Messenger Ribosomes/drug effects,metabolism Rifampin/pharmacology Spectrophotometry Sucrose Tritium Ultrasonics Vibration

Chemicals

Amino Acids Bacterial Proteins Carbon Isotopes Culture Media RNA, Bacterial RNA, Messenger Tritium Sucrose Rifampin

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Ron E Z

References (15)

15 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.108.1.263-268.1971

Published

1971-10-00

Pages

263-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247060

Subset

External Links

PubMed source DOI: 10.1128/jb.108.1.263-268.1971 PMC Full Text View journal details

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Polysome turnover during amino acid starvation in Escherichia coli.

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