Chromatography of nucleic acid digests on thin layers of cellulose impregnated with polyethyleneimine.

The Biochemical journal ·Vol. 123 ·No. 4 ·1971-07-00 ·Pages 613-7

Southern EM, Mitchell AR

Abstract

Methods are described for the separation of oligodeoxyribonucleotides and oligoribonucleotides by chromatography on thin layers of cellulose impregnated with polyethyleneimine.

MeSH Terms

Animals Cellulose Chromatography, Thin Layer DNA, Bacterial/analysis Deoxyribonucleotides/analysis Escherichia coli/analysis Imines Methods Nucleic Acids/analysis Phosphorus Isotopes Polyethylenes Proteus/analysis Pyrimidines/analysis RNA, Bacterial/analysis RNA, Ribosomal/analysis Ribonucleases Ribonucleotides/analysis Xenopus

Chemicals

DNA, Bacterial Deoxyribonucleotides Imines Nucleic Acids Phosphorus Isotopes Polyethylenes Pyrimidines RNA, Bacterial RNA, Ribosomal Ribonucleotides Cellulose Ribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Southern E M

Mitchell A R

References (9)

9 references, click to expand

A two-dimensional fractionation procedure for radioactive nucleotides.
J Mol Biol. 1965 Sep;13(2):373-98 PMID: 5325727
Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.
Eur J Biochem. 1969 Dec;11(2):395-9 PMID: 4902612
Nucleotide sequences present within the 16S ribosomal RNA of Escherichia coli.
Nature. 1970 Jan 3;225(5227):26-9 PMID: 4903099
Selective inhibition of a nuclear RNA polymerase in cells infected with frog virus.
Nature. 1970 Jul 18;227(5255):294-5 PMID: 5428203
The fractionation of RNA by polyacrylamide gel electrophoresis.
Methods Med Res. 1970;12:359-68 PMID: 5432494
Nucleotide 'maps' of digests of deoxyribonucleic acid.
Biochem J. 1970 Aug;118(5):831-41 PMID: 5476726
The separation of pyrimidine oligonucleotides obtained from deoxyribonucleic acid.
Biochim Biophys Acta. 1961 Jul 22;51:212-4 PMID: 13734807
STUDIES ON THE NUCLEOTIDE ARRANGEMENT IN DEOXYRIBONUCLEIC ACIDS. VII. DIRECT ESTIMATION OF PYRIMIDINE NUCLEOTIDE RUNS.
Biochim Biophys Acta. 1963 Sep 17;76:1-8 PMID: 14068557
CHROMATOGRAPHY OF RIBONUCLEASE T1 DIGESTS OF RNA ON THE DEAE-CELLULOSE OF 7 M UREA.
Biochemistry. 1963 Nov-Dec;2:1179-84 PMID: 14099608

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1230613

Published

1971-07-00

Pages

613-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177002

Subset

External Links

PubMed source DOI: 10.1042/bj1230613 PMC Full Text View journal details

Analysis Services

Chromatography of nucleic acid digests on thin layers of cellulose impregnated with polyethyleneimine.

Contact

WeChat Official Account