Home LiteratureArticle Details
PMID: 4942912 Published · ppublish English Journal Article

On the control of the induction of -galactosidase in synchronous cultures of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 8 ·1971-08-00 ·Pages 1702-6

Goldberg RB, Chargaff E

Abstract

The mode of induction of beta-galactosidase (EC 3.2.1.23) in synchronously growing cultures of two Hfr strains of Escherichia coli K12 was investigated. Cells can be induced to form the enzyme during any portion of the cell cycle; but when they are grown permanently in the presence of an inducer, enzyme synthesis is discontinuous. The interruption of beta-galactosidase synthesis appears to be geared to the growth cycle: it occurs when the cells are dividing actively. The observation that lac-specific messenger RNA is produced also in the absence of detectable enzyme synthesis suggests the existence of a control mechanism operating on the level of translation.

MeSH Terms
Bacterial Proteins/biosynthesis Cell Division Culture Media DNA, Bacterial/biosynthesis Enzyme Induction Escherichia coli/enzymology,growth & development,metabolism Galactose/pharmacology Galactosidases/biosynthesis Glycosides/pharmacology Nucleic Acid Hybridization RNA, Bacterial/biosynthesis RNA, Messenger/biosynthesis Tritium Uracil/metabolism
Chemicals
Bacterial Proteins Culture Media DNA, Bacterial Glycosides RNA, Bacterial RNA, Messenger Tritium Uracil Galactosidases Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goldberg R B
Chargaff E
References (27)
27 references, click to expand
  1. Sequence of enzyme synthesis and gene replication during the cell cycle of Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1965 Jul;54(1):64-70 PMID: 4955131
  2. Genetic implications of periodic pulsations of the rate of synthesis and the composition of rapidly labeled bacterial RNA.
    Proc Natl Acad Sci U S A. 1965 Sep;54(3):904-11 PMID: 5324400
  3. Evidence for polarity of chromosome replication in F- strains of Escherichia coli.
    Genet Res. 1966 Aug;8(1):119-24 PMID: 5329974
  4. Beta-galactosidase formation controlled by an episomal gene during the cell cycle of Escherichia coli.
    J Biochem. 1966 Sep;60(3):338-40 PMID: 5339377
  5. Chain initiation in a polycistronic message: sequential versus simultaneous derepression of the enzymes for histidine biosynthesis in Salmonella typhimurium.
    Proc Natl Acad Sci U S A. 1967 Jun;57(6):1857-64 PMID: 5340637
  6. Replication of the escherichia coli K12 chromosome.
    Proc Natl Acad Sci U S A. 1967 Nov;58(5):1911-8 PMID: 4866982
  7. Enzyme and nucleic acid formation during synchronous growth of Rhodopseudomonas spheroides.
    J Bacteriol. 1968 Apr;95(4):1400-6 PMID: 5650892
  8. The lac operator is DNA.
    Proc Natl Acad Sci U S A. 1967 Dec;58(6):2415-21 PMID: 4873589
  9. Studies on the size of the messenger-RNA transcribed from the histidine operon during simultaneous and sequential depression.
    Biochim Biophys Acta. 1968 Aug 23;166(1):124-33 PMID: 4880556
  10. Stimulation of tryptophanase synthesis in Escherichia coli by cyclic 3',5'-adenosine monophosphate.
    J Biol Chem. 1969 Apr 25;244(8):2226-32 PMID: 4305668
  11. Kinetics of induction of the lactose operon on an episome in Samonellla typhimurium.
    J Bacteriol. 1969 Jun;98(3):1232-8 PMID: 4892373
  12. The stimulatory effect of cyclic adenosine 3'5'-monophosphate on DNA-directed synthesis of beta-galactosidase in a cell-free system.
    Proc Natl Acad Sci U S A. 1969 May;63(1):118-22 PMID: 4309055
  13. Chromosome replication in Escherichia coli. II. Origin of replication in F- and F+ strains.
    J Mol Biol. 1969 Oct 28;45(2):325-36 PMID: 4904407
  14. Selective translation of T4 template RNA by ribosomes from T4-infected Escherichia coli.
    Proc Natl Acad Sci U S A. 1969 Sep;64(1):345-51 PMID: 4904642
  15. Control of translation by T4 phage: altered binding of disfavoured messengers.
    Nature. 1970 May 30;226(5248):820-3 PMID: 5444623
  16. Control of translation of T4 phage: altered ribosome binding at R17 initiation sites.
    Nature. 1970 May 30;226(5248):824-7 PMID: 5444624
  17. Mechanism of activation of catabolite-sensitive genes: a positive control system.
    Proc Natl Acad Sci U S A. 1970 May;66(1):104-10 PMID: 4320461
  18. Translational repression in the arginine system of Escherichia coli.
    Proc Natl Acad Sci U S A. 1970 Dec;67(4):1703-9 PMID: 4923118
  19. Inhibition of host protein synthesis during infection of Escherichia coli by bacteriophage T4. II. Induction of host messenger ribonucleic acid and its exclusion from polysomes.
    J Virol. 1970 Aug;6(2):208-17 PMID: 4925774
  20. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  21. Biochemical aspects of the cell growth of Escherichia coli as studied by the method of synchronous culture.
    J Bacteriol. 1956 Dec;72(6):821-6 PMID: 13398371
  22. Genetic regulatory mechanisms in the synthesis of proteins.
    J Mol Biol. 1961 Jun;3:318-56 PMID: 13718526
  23. The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg.
    J Mol Biol. 1960 Dec;2:392-415 PMID: 13750787
  24. Biosynthesis of B-D-galactosidase controlled by phage-carried genes. I. Induced beta-D-galactosidase biosynthesis after transduction of gene z-plus by phage.
    Proc Natl Acad Sci U S A. 1961 Dec 15;47:1956-67 PMID: 14491633
  25. RHYTHMIC ALTERNATIONS IN THE RATE OF SYNTHESIS AND THE COMPOSITION OF RAPIDLY LABELLED RIBONUCLEIC ACID DURING THE SYNCHRONOUS GROWTH OF BACTERIA.
    Nature. 1964 Aug 1;203:479-83 PMID: 14202364
  26. MACROMOLECULAR SYNTHESIS DURING SYNCHRONOUS GROWTH OF ESCHERICHIA COLI B/R.
    Biochim Biophys Acta. 1965 Feb 8;95:341-50 PMID: 14293708
  27. POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES.
    Nature. 1965 Apr 3;206:93 PMID: 14334364
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.8.1702
Published
1971-08-00
Pages
1702-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389275
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.8.1702 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2