Home LiteratureArticle Details
PMID: 4943549 Published · ppublish English Journal Article

New polarity suppressors in Escherichia coli: suppression and messenger RNA stability.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 12 ·1971-12-00 ·Pages 2962-6

Carter T, Newton A

Abstract

Two new polarity suppressors (Su27 and Su78) have been isolated in Escherichia coli. Both suppress polarity in the trp and lac operons, and neither shows codon specificity for suppression. Extreme polar mutants of the lac z gene that contain either Su27 or Su78 make mRNA from the entire operon; however, no active beta-galactosidase is translated from the z-gene messenger, and the amount of distal messenger is greater than would be expected from corresponding enzyme activities. Puromycin treatment of lac(+) strains mimics the effect of polar chain-termination mutations in destabilizing lac mRNA. This effect is completely reversed by Su27, and it is partially reversed by Su78. The results suggest that these suppressors act by stabilizing mRNA after premature termination of protein synthesis.

MeSH Terms
Acyltransferases/metabolism Alleles Cells, Cultured Drug Stability Escherichia coli/enzymology,metabolism Galactose Galactosidases/metabolism Genetic Code Lactose/biosynthesis Membrane Transport Proteins/metabolism Mutation Nucleic Acid Denaturation/drug effects Nucleic Acid Hybridization Operon Puromycin/pharmacology RNA, Bacterial/metabolism RNA, Messenger/metabolism Rifampin/pharmacology Sulfhydryl Compounds Suppression, Genetic Time Factors Tritium Tryptophan/biosynthesis Uridine
Chemicals
Membrane Transport Proteins RNA, Bacterial RNA, Messenger Sulfhydryl Compounds Tritium Puromycin Tryptophan Acyltransferases Galactosidases Lactose Rifampin Uridine Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Carter T
Newton A
References (26)
26 references, click to expand
  1. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
    J Mol Biol. 1965 Jul;12(3):829-42 PMID: 4955314
  2. A SECOND PERMEASE FOR METHYL-THIO-BETA-D-GALACTOSIDE IN ESCHERICHIA COLI.
    Biochim Biophys Acta. 1965 May 4;100:591-3 PMID: 14347955
  3. Increased stability of polysomes in an Escherichia coli mutant with relaxed control of RNA synthesis.
    Proc Natl Acad Sci U S A. 1966 Aug;56(2):471-5 PMID: 5338826
  4. Effect of nonsense mutations on translation of the lactose operon of Escherichia coli.
    Cold Spring Harb Symp Quant Biol. 1966;31:181-7 PMID: 4866374
  5. Polarity in the tryptophan operon of E. coli.
    Cold Spring Harb Symp Quant Biol. 1966;31:235-49 PMID: 4866380
  6. Mutational alteration of the maximal level of Lac operon expression.
    Cold Spring Harb Symp Quant Biol. 1966;31:403-8 PMID: 4866390
  7. The formation and stabilization of 30S and 50S ribosome couples in Escherichia coli.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1782-9 PMID: 4867673
  8. The periodicity of RNA polymerase initiations: a new regulatory feature of transcription.
    Proc Natl Acad Sci U S A. 1968 May;60(1):313-20 PMID: 4872442
  9. Messenger RNA stability and polarity in the Lac operon of Escherichia coli.
    Nature. 1969 Aug 16;223(5207):707-10 PMID: 4895877
  10. Re-initiation of polypeptide synthesis and polarity in the lac operon of Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):329-39 PMID: 4896019
  11. Polarity and the degradation of mRNA.
    Nature. 1969 Oct 25;224(5217):329-31 PMID: 4898925
  12. Isolation of pure lac operon DNA.
    Nature. 1969 Nov 22;224(5221):768-74 PMID: 4902927
  13. Orientation of transcription of the lac operon and its repressor gene in Escherichia coli.
    J Mol Biol. 1969 Feb 28;40(1):145-51 PMID: 4903358
  14. Relief of polarity in E. coli by "suA".
    Nature. 1970 Apr 4;226(5240):28-31 PMID: 4907561
  15. Isolation and characterization of frameshift mutations in the lac operon.
    J Mol Biol. 1970 May 14;49(3):589-601 PMID: 4916550
  16. Evidence for premature termination of transcription of the tryptophan operon in polarity mutants of Escherichia coli.
    Nature. 1970 Oct 17;228(5268):232-5 PMID: 4920918
  17. Regulation of lac transcription in antibiotic-treated E. coli.
    Nat New Biol. 1971 Mar 10;230(10):41-4 PMID: 4324113
  18. Untranslated T7 phage mRNA is stabilized in suA host.
    Nat New Biol. 1971 Apr 14;230(15):208 PMID: 4929518
  19. Loss of dispensable endonuclease activity in relief of polarity by suA.
    Nat New Biol. 1971 Jun 16;231(24):214-7 PMID: 4932570
  20. Level of tryptophan messenger RNA in Escherichia coli.
    J Mol Biol. 1968 Oct 28;37(2):289-302 PMID: 4939045
  21. Transduction by bacteriophage P-1 and the properties of the lac genetic region in E. coli and S. dysenteriae.
    Virology. 1961 Nov;15:299-311 PMID: 13894683
  22. Synthesis and turnover of phage messenger RNA in E. coli infected with bacteriophage T4 in the presence of chloromycetin.
    J Mol Biol. 1962 Nov;5:527-34 PMID: 13939775
  23. RESTORATION OF OPERON ACTIVITY BY SUPPRESSORS.
    Biochim Biophys Acta. 1963 Sep 17;76:162-4 PMID: 14068553
  24. RECOMBINATION BETWEEN COLIPHAGES LAMBDA AND PHI-80.
    Virology. 1964 Apr;22:650-1 PMID: 14166126
  25. A NONSPECIFIC INCREASE IN PERMEABILITY IN ESCHERICHIA COLI PRODUCED BY EDTA.
    Proc Natl Acad Sci U S A. 1965 Apr;53:745-50 PMID: 14324530
  26. Nonsense mutants and polarity in the lac operon of Escherichia coli.
    J Mol Biol. 1965 Nov;14(1):290-6 PMID: 5327654
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.12.2962
Published
1971-12-00
Pages
2962-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389570
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.12.2962 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2