Structure of the replicating DNA from bacteriophage T4.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 12 ·1971-12-00 ·Pages 3049-53

Delius H, Howe C, Kozinski AW

Abstract

At an early stage of replication, parental T4 DNA shows a loop structure often displaying two 3'-ended, single-stranded "whiskers", located in trans configuration at the branching-points. Several such loops have been observed within a single T4 molecule. Occasionally, reinitiation occurred in the middle of a loop, which suggests that the loop was growing in both directions.

MeSH Terms

Centrifugation, Density Gradient Coliphages/analysis DNA Replication DNA, Bacterial/analysis Deoxyribonucleases Escherichia coli/analysis Microscopy, Electron Models, Structural Nucleic Acid Conformation Phosphorus Isotopes Tritium

Chemicals

DNA, Bacterial Phosphorus Isotopes Tritium Deoxyribonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Delius H

Howe C

Kozinski A W

References (11)

11 references, click to expand

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Early intracellular events in the replication T4 phage DNA. II. Partially replicated DNA.
Proc Natl Acad Sci U S A. 1965 Aug;54(2):634-40 PMID: 5218656
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A physical study by electron microscopy of the terminally reptitious, circularly permuted DNA from the coliphage particles of Escherichia coli 15.
J Mol Biol. 1970 Feb 28;48(1):1-22 PMID: 4915293
Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. V. Further studies on the T4 protein-deoxyribonucleic acid complex.
J Virol. 1970 Apr;5(4):490-501 PMID: 4916323
Position of branch points in replicating lambda DNA.
J Mol Biol. 1970 Jul 14;51(1):61-73 PMID: 5481280
A preferred origin and direction of bacteriophage T4 DNA replication. I. A gradient of allele frequencies in crosses between normal and small T4 particles.
J Mol Biol. 1970 Nov 14;53(3):503-14 PMID: 4924010
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J Virol. 1967 Aug;1(4):758-70 PMID: 5621474

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.12.3049

Published

1971-12-00

Pages

3049-53

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389588

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.12.3049 PMC Full Text View journal details

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Structure of the replicating DNA from bacteriophage T4.

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