Use of yeast populations fractionated by zonal centrifugation to study the cell cycle.

Journal of bacteriology ·Vol. 108 ·No. 3 ·1971-12-00 ·Pages 1045-50

Sebastian J, Carter BL, Halvorson HO

Abstract

Zonal centrifugation in a sucrose density gradient was used to separate yeast cells primarily by size and thus by age in the cell cycle. This approach provides an alternative to synchronous growth for examining the properties of cells at different stages in the cell cycle.

MeSH Terms

Cell Division Centrifugation, Density Gradient Centrifugation, Zonal Culture Media DNA/biosynthesis Galactosidases/biosynthesis Glucosidases/biosynthesis Ornithine RNA/biosynthesis Saccharomyces/cytology,enzymology,growth & development,isolation & purification,metabolism Saccharomyces cerevisiae/enzymology,growth & development,isolation & purification,metabolism Sucrose Transaminases/biosynthesis

Chemicals

Culture Media Sucrose RNA DNA Ornithine Transaminases Galactosidases Glucosidases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sebastian J

Carter B L

Halvorson H O

References (13)

13 references, click to expand

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Nature. 1967 Jun 3;214(5092):983-6 PMID: 4861634
Enzyme synthesis in synchronous cultures.
Science. 1969 Aug 15;165(3894):657-63 PMID: 4893637
Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures.
J Bacteriol. 1970 Dec;104(3):1280-5 PMID: 16559104
The growth of single cells. II. Saccharomyces cerevisiae.
Exp Cell Res. 1958 Aug;15(1):214-21 PMID: 13574174
THE PATHWAY OF ARGININE BREAKDOWN IN SACCHAROMYCES CEREVISIAE.
Biochim Biophys Acta. 1964 Dec 9;93:650-2 PMID: 14263163
The purification and properties of an alpha-glucosidase of Saccharomyces italicus Y1225.
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The nucleic acids of plant tissues; the extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid.
Arch Biochem. 1950 Feb;25(2):262-76 PMID: 15404816
Onset of DNA synthesis during the cell cycle in chemostat cultures.
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1611-7 PMID: 5340631
Synchronization of cell division.
Bacteriol Rev. 1957 Dec;21(4):263-72 PMID: 13488884
Evidence for the generality of linear cell growth.
J Theor Biol. 1970 Jul;28(1):15-29 PMID: 4919340
The use of conventional and zonal centrifugation to study the life cycle of mammalian cells. Phospholipid and macromolecular synthesis in neoplastic mast cells.
Biochem J. 1970 Sep;119(3):493-9 PMID: 5500309
The behaviour of nucleic acids in synchronously dividing cultures of Saccharomyces cerevisiae.
Exp Cell Res. 1960 Aug;20:338-49 PMID: 13844952
The development of low-speed "A" series zonal rotors.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.108.3.1045-1050.1971

Published

1971-12-00

Pages

1045-50

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247186

Subset

External Links

PubMed source DOI: 10.1128/jb.108.3.1045-1050.1971 PMC Full Text View journal details

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Use of yeast populations fractionated by zonal centrifugation to study the cell cycle.

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