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PMID: 4958772 Published · ppublish English Journal Article

Heat-labile antigens of Salmonella enteritidis. I. Extraction of antigens.

Journal of bacteriology ·Vol. 92 ·No. 3 ·1966-09-00 ·Pages 543-8

Milne M, Collins FM

Abstract

Milne, Margaret (University of Adelaide, Adelaide, Australia), and F. M. Collins. Heat-labile antigens of Salmonella enteritidis. I. Extraction of antigens. J. Bacteriol. 92:543-548. 1966.-Salmonella enteritidis strains of high and low mouse virulence were grown in continuous culture. Cell walls were obtained from both strains by sonic disruption, and the washed walls were extracted at 4 C with sodium dodecyl sulfate or sodium deoxycholate. The extracts were eluted with a saline gradient from a diethylaminoethyl cellulose column, and the separated peaks were tested for precipitin activity against rabbit antisera prepared with heator ethyl alcohol-killed vaccines. The separated antigens reacted less intensely with the antiserum to heat-killed cells than they did to the antisera prepared against alcohol-killed vaccine. Little antigenic difference could be detected between the extracts prepared from the virulent and the avirulent organisms.

MeSH Terms
Animals Antigens/analysis Bile Acids and Salts Cellulose Chemical Phenomena Chemistry Chromatography, Thin Layer Culture Media Detergents Immune Sera Immunodiffusion Mice Precipitins/analysis Rabbits Salmonella/immunology Spectrophotometry Virulence
Chemicals
Antigens Bile Acids and Salts Culture Media Detergents Immune Sera Precipitins Cellulose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Milne M
Collins F M
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20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.92.3.543-548.1966
Published
1966-09-00
Pages
543-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC276287
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.92.3.543-548.1966 PMC Full Text View journal details
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