Rapid quantitative serological assay of staphylococcal enterotoxin B.

Applied microbiology ·Vol. 14 ·No. 2 ·1966-03-00 ·Pages 284-91

Weirether FJ, Lewis EE, Rosenwald AJ, Lincoln RE

Abstract

A simple, rapid method, based on the Oudin single diffusion technique, is described for the quantitative assay of staphylococcal enterotoxin B. The method yields reproducible results without close control of such assay variables as temperature, antiserum dilution, and assay time, provided that the ionic strength is maintained above 0.2 n sodium chloride equivalent.

MeSH Terms

Enterotoxins Hydrogen-Ion Concentration Immune Sera Immunodiffusion Sodium Chloride Staphylococcus Temperature Toxins, Biological

Chemicals

Enterotoxins Immune Sera Toxins, Biological Sodium Chloride

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Weirether F J

Lewis E E

Rosenwald A J

Lincoln R E

References (5)

5 references, click to expand

B. Specific precipitation in gels and its application to immunochemical analysis.
Methods Med Res. 1952;5:335-78 PMID: 13002126
Purification of staphylococcal enterotoxin B.
Biochemistry. 1965 Jun;4(6):1011-6 PMID: 4953912
Quantitative antigen analysis by the Oudin method.
J Immunol. 1954 Nov;73(5):322-30 PMID: 13212054
QUANTITATIVE DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN B IN FOOD BY GEL-DIFFUSION METHODS.
Public Health Rep. 1963 Dec;78:1089-98 PMID: 14090082
SEROLOGICAL ASSAY OF CULTURE FILTRATES FOR STAPHYLOCOCCUS ENTEROTOXIN.
J Bacteriol. 1963 Apr;85:955-6 PMID: 14044975

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.14.2.284-291.1966

Published

1966-03-00

Pages

284-91

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC546670

Subset

External Links

PubMed source DOI: 10.1128/am.14.2.284-291.1966 PMC Full Text

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