Home LiteratureArticle Details
PMID: 4960918 Published · ppublish English Journal Article

Nature of the repair of methyl methanesulfonate-induced damage in Bacillus subtilis.

Journal of bacteriology ·Vol. 93 ·No. 3 ·1967-03-00 ·Pages 1056-62

Reiter H, Strauss B, Robbins M, Marone R

Abstract

A nuclease present in extracts of Bacillus subtilis inserts breaks in deoxyribonucleic acid (DNA) treated with the monofunctional alkylating agent, methyl methanesulfonate (MMS), but the nature of the sites within the alkylated macromolecule at which these breaks occur is not known. DNA extracted from B. subtilis cells that have recovered from MMS damage has lost its susceptibility to enzyme action. The recovery process is accompanied by some DNA breakdown and by the incorporation of thymidine. Some recovery from ultraviolet irradiation (UV) and MMS occurred in organisms starved for thymine or adenine, but UV recovery was stimulated by their addition. It is possible that MMS recovery proceeds by a process of excision and repair similar to, but not identical with, UV repair.

MeSH Terms
Alkylating Agents/pharmacology Bacillus subtilis/drug effects,enzymology,radiation effects DNA, Bacterial/metabolism Deoxyribonucleases/metabolism Nucleic Acid Denaturation Nucleic Acids Ultraviolet Rays
Chemicals
Alkylating Agents DNA, Bacterial Nucleic Acids Deoxyribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Reiter H
Strauss B
Robbins M
Marone R
References (22)
22 references, click to expand
  1. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM.
    Proc Natl Acad Sci U S A. 1964 Feb;51:226-31 PMID: 14124320
  2. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
    J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
  3. Integration of deoxyribonuclease-treated DNA in bacillus subtilis transformation.
    J Gen Physiol. 1966 Jul;49(6):233-58 PMID: 4961186
  4. DARK REPAIR OF ULTRAVIOLET INJURY IN E. COLI DURING DEPRIVATION OF THYMINE.
    Nature. 1964 May 9;202:614-5 PMID: 14195082
  5. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.
    J Biol Chem. 1961 May;236:1372-9 PMID: 13767412
  6. Distribution density of nucleotides within a desoxyribonucleic acid chain.
    J Biol Chem. 1953 Aug;203(2):673-88 PMID: 13084637
  7. EFFECTS OF ALKYLATING AGENTS ON T2 AND T4 BACTERIOPHAGES.
    Biochem J. 1963 Oct;89:138-44 PMID: 14100794
  8. Recovery from ultraviolet- and alkylating-agent-induced damage in Bacillus subtilis.
    Radiat Res. 1966;:Suppl 6:201+ PMID: 4959725
  9. DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes.
    Radiat Res. 1966;:Suppl 6:156+ PMID: 5334390
  10. INTERRUPTIONS IN THE POLYNUCLEOTIDE STRANDS IN BACTERIOPHAGE DNA.
    J Mol Biol. 1964 Jan;8:1-10 PMID: 14149959
  11. Actinomycin D inhibition of UV-dark repair in Bacillus subtilis.
    Biochem Biophys Res Commun. 1966 Sep 22;24(6):892-8 PMID: 4961532
  12. Differential destruction of the transforming activity of damaged deoxyribonucleic acid by a bacterial enzyme.
    Proc Natl Acad Sci U S A. 1962 Sep 15;48:1670-5 PMID: 13917693
  13. Repair of DNA studied with a nuclease specific for UV-induced lesions.
    Proc Natl Acad Sci U S A. 1966 Sep;56(3):932-9 PMID: 4961546
  14. Relation of the repair of damage induced by a monofunctional alkylating agent to the repair of damage induced by ultraviolet light in Bacillus subtilis.
    Biochem Biophys Res Commun. 1965 Sep 22;20(6):680-7 PMID: 4955535
  15. Recovery of deoxyribonucleic acid from the effects of alkylation.
    J Gen Microbiol. 1963 Jan;30:89-103 PMID: 13978795
  16. EVIDENCE FOR REPAIR-REPLICATION OF ULTRAVIOLET DAMAGED DNA IN BACTERIA.
    J Mol Biol. 1964 Aug;9:395-410 PMID: 14202275
  17. The excision of thymine dimer from the DNA of UV-irradiated E. coli 15 T-A-U during thymine deprivation.
    Biochem Biophys Res Commun. 1964 Jul 27;16(5):489-96 PMID: 5332853
  18. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12.
    Proc Natl Acad Sci U S A. 1964 Feb;51:293-300 PMID: 14124327
  19. Molecular mechanism of the cytotoxic action of difunctional alkylating agents and of resistance to this action.
    Nature. 1965 May 1;206(983):480-3 PMID: 5319105
  20. Mechanism of photoinactivation of coliphage T-7 sensitized by acridine orange.
    Virology. 1966 Sep;30(1):97-103 PMID: 5911894
  21. Repair of damage induced by a monofunctional alkylating agent in a transformable, ultraviolet-sensitive strain of Bacillus subtilis.
    J Mol Biol. 1965 Nov;14(1):179-94 PMID: 4956865
  22. The reaction of mono- and di-functional alkylating agents with nucleic acids.
    Biochem J. 1961 Sep;80(3):496-503 PMID: 16748923
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.93.3.1056-1062.1967
Published
1967-03-00
Pages
1056-62
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC276553
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.93.3.1056-1062.1967 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2