Enharncement of the hemolytic activity of the second component of human complement by oxidation.

The Journal of experimental medicine ·Vol. 126 ·No. 6 ·1967-12-01 ·Pages 1013-25

Polley MJ, Müller-Eberhard HJ

Abstract

A method has been described for the chemical modification of human C'2 which results in a pronounced enhancement of its hemolytic activity and a marked increase in the stability of the intermediate complex EAC'1a,4,2a prepared with the modified C'2. Both effects are fully explained by the observed increase in activity and stability of the C'3 converting enzyme, C'4,2a, following its generation with modified C'2. Evidence has been presented in support of the hypothesis that the modification resulting from treatment of C'2 with a critical concentration of iodine consists of oxidation of one or more sulfhydryl group within the molecule.

MeSH Terms

Animals Biological Assay Complement System Proteins/analysis,physiology Cysteine/pharmacology Edetic Acid/pharmacology Esterases/analysis Glutathione/pharmacology Hemolysis/drug effects Humans Immunochemistry In Vitro Techniques Iodine/pharmacology Iodoacetates/pharmacology Mercaptoethanol/pharmacology Oxidation-Reduction Rabbits Sheep Sulfhydryl Compounds/pharmacology

Chemicals

Iodoacetates Sulfhydryl Compounds Mercaptoethanol Complement System Proteins Iodine Edetic Acid Esterases Glutathione Cysteine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Polley M J

Müller-Eberhard H J

References (14)

14 references, click to expand

Formation and functional significance of a molecular complex derived from the second and the fourth component of human complement.
J Exp Med. 1967 Feb 1;125(2):359-80 PMID: 6019133
Concerning the mechanism of complement action. V. The early steps in immune hemolysis.
J Immunol. 1960 Mar;84:299-308 PMID: 13798073
ISOLATION OF BETA IF-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT.
J Exp Med. 1965 Aug 1;122:277-98 PMID: 14316946
ISOLATION AND DESCRIPTION OF THE FOURTH COMPONENT OF HUMAN COMPLEMENT.
J Exp Med. 1963 Sep 1;118:447-66 PMID: 14078003
STUDIES ON HUMAN C'1-ESTERASE. I. PURIFICATION AND ENZYMATIC PROPERTIES.
J Immunol. 1964 Mar;92:456-67 PMID: 14128991
The oxidation of protein thiol groups by iodate, bromate and persulphate.
Biochem J. 1961 Sep;80:612-6 PMID: 13714532
Carboxypeptidase A: approaches to the chemical nature of the active center and the mechanisms of action.
Proc Natl Acad Sci U S A. 1963 Jan 15;49:109-16 PMID: 13995911
The chemistry of iodination.
Ann N Y Acad Sci. 1957 Aug 30;70(1):3-18 PMID: 13488250
ACTIVE CENTER OF CARBOXYPEPTIDASE A.
Fed Proc. 1964 Jan-Feb;23:8-17 PMID: 14117872
Kinetic studies on immune hemolysis. VII. Decay of EAC'1, 4, 2, fixation of C'3, and other factors influencing the hemolytic action of complement.
J Immunol. 1954 Dec;73(6):443-54 PMID: 13212068
Chromatographic resolution of the first component of human complement into three activities.
J Exp Med. 1963 Jun 1;117:983-1008 PMID: 13929797
C'2ad, an inactive derivative of C'2 released during decay of EAC'4,2a.
Immunochemistry. 1966 May;3(3):163-76 PMID: 5957242
COMPLEMENT: INACTIVATION OF SECOND COMPONENT BY P-HYDROXYMERCURIBENZOATE.
Science. 1965 Feb 26;147(3661):1034-5 PMID: 14245776
Analysis of modified beta-lactoglobulins and ovalbumins prepared from the sulfenyl iodine intermediates.
J Biol Chem. 1960 Jun;235:1711-5 PMID: 13813314

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.126.6.1013

Published

1967-12-01

Pages

1013-25

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2138419

Subset

External Links

PubMed source DOI: 10.1084/jem.126.6.1013 PMC Full Text View journal details

Analysis Services

Enharncement of the hemolytic activity of the second component of human complement by oxidation.

Contact

WeChat Official Account