The oxidation of cyst(e)ine by mast-cell tumour P815 in culture.

The Biochemical journal ·Vol. 106 ·No. 2 ·1968-01-00 ·Pages 437-44

Wheldrake JF, Pasternak CA

Abstract

1. Mast-cell tumour P815 cells oxidize [(35)S]cyst(e)ine to (35)SO(4) (2-). 2. Addition of cysteinesulphinate or sulphite decreases the formation of (35)SO(4) (2-); at the same time [(35)S]cysteinesulphinate or (35)SO(3) (2-) accumulates. 3. Extracts of P815 cells form sulphite from cysteinesulphinate and 2-oxoglutarate. The K(m) for cysteinesulphinate is 6.35mm and that for 2-oxoglutarate is 0.165mm. 4. Extracts oxidize sulphite to sulphate. 5. No formation of hydrogen sulphide from cyst(e)ine was detectable. 6. It is concluded that P815 cells oxidize cyst(e)ine to sulphate solely via cysteinesulphinate and sulphite. 7. The concentration of the enzymes catalysing this sequence is unaltered by several variations in the conditions of growth.

MeSH Terms

Animals Chromatography, Paper Culture Techniques Cysteine/metabolism Cystine/metabolism Hydrogen-Ion Concentration Liver/metabolism Mast-Cell Sarcoma/metabolism Mice Oxidation-Reduction Sarcoma, Experimental/metabolism Sulfates/metabolism Sulfinic Acids/metabolism Sulfites/metabolism Sulfur Isotopes

Chemicals

Sulfates Sulfinic Acids Sulfites Sulfur Isotopes Cystine Cysteine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Wheldrake J F

Pasternak C A

References (13)

13 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1060437

Published

1968-01-00

Pages

437-44

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1198521

Subset

External Links

PubMed source DOI: 10.1042/bj1060437 PMC Full Text View journal details

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The oxidation of cyst(e)ine by mast-cell tumour P815 in culture.

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