Deoxyribonucleic acid degradation in Bacillus subtilis during exposure to actinomycin D.

Journal of bacteriology ·Vol. 95 ·No. 4 ·1968-04-00 ·Pages 1450-60

Farmer JL

Abstract

At high concentrations (10 mug/ml), actinomycin D inhibited deoxyribonucleic acid (DNA) synthesis in Bacillus subtilis. Inhibition occurred quickly (in less than 1 min) and was complete. In strain 23 thy his, inhibition of DNA synthesis by actinomycin D was followed by partial degradation of one of the two daughter strands to acid-soluble products. Degradation began at the replication point and proceeded over a distance equal to about 12% of a chromosome in length. Actinomycin D played some essential part in degradation, since exposure of the cells to other treatments or agents which inhibit growth did not lead to the above result.

MeSH Terms

Acridines/pharmacology Anti-Bacterial Agents/pharmacology Bacillus subtilis/drug effects,metabolism Carbon Isotopes Centrifugation, Density Gradient Chromosomes, Bacterial/drug effects DNA, Bacterial/biosynthesis,metabolism Dactinomycin/pharmacology Models, Theoretical Nitrogen/pharmacology RNA, Bacterial/biosynthesis Radiometry Thymine/metabolism Tritium

Chemicals

Acridines Anti-Bacterial Agents Carbon Isotopes DNA, Bacterial RNA, Bacterial Tritium Dactinomycin Nitrogen Thymine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Farmer J L

References (26)

26 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.95.4.1450-1460.1968

Published

1968-04-00

Pages

1450-60

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC315106

Subset

External Links

PubMed source DOI: 10.1128/jb.95.4.1450-1460.1968 PMC Full Text View journal details

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Deoxyribonucleic acid degradation in Bacillus subtilis during exposure to actinomycin D.

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