Microcycle sporogenesis of Bacillus cereus in a chemically defined medium.

Journal of bacteriology ·Vol. 95 ·No. 2 ·1968-02-00 ·Pages 355-9

MacKechnie I, Hanson RS

Abstract

A chemically defined medium which allowed germination, outgrowth, and subsequent resporulation of Bacillus cereus T spores, without intervening cell division (microcycle sporogenesis), is described. No medium replacement was required. The second-stage spores were heat-stable and had similar germination characteristics and dipicolinic acid content to primary spores. Deoxyribonucleic acid (DNA) replication began soon after germination and there was a doubling in the DNA content of the cells within 2 hr.

MeSH Terms

Bacillus cereus/drug effects,growth & development Culture Media DNA, Bacterial/metabolism Dactinomycin/pharmacology Mitomycins/pharmacology Phosphates/pharmacology Picolinic Acids/analysis Spores/growth & development Thymidine/metabolism Tritium Uracil/metabolism

Chemicals

Culture Media DNA, Bacterial Mitomycins Phosphates Picolinic Acids Tritium Dactinomycin Uracil Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

MacKechnie I

Hanson R S

References (12)

12 references, click to expand

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J Bacteriol. 1967 Aug;94(2):441-57 PMID: 4962705
Metabolic requirements for microcycle sporogenesis of Bacillus megaterium.
J Bacteriol. 1967 Aug;94(2):434-40 PMID: 4962704
Direct Transition of Outgrowing Bacterial Spores to New Sporangia Without Intermediate Cell Division.
J Bacteriol. 1965 Sep;90(3):803-7 PMID: 16562084
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
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Development of fine structure, thermostability, and dipicolinate during sporogenesis in a bacillus.
Can J Microbiol. 1960 Apr;6:203-12 PMID: 13851956
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J Gen Microbiol. 1966 Sep;44(3):419-37 PMID: 5339419
Biochemistry of sporulation. I. Metabolism of acetate by vegetative and sporulating cells.
J Bacteriol. 1963 Feb;85:451-60 PMID: 13952646
Colorimetric assay for dipicolinic acid in bacterial spores.
Science. 1958 Jan 3;127(3288):26-7 PMID: 13495474
Developmental changes during the formation and breaking of the dormant state in bacteria.
Annu Rev Microbiol. 1966;20:169-88 PMID: 5330231
Biochemical changes occurring during sporulation of Bacillus cereus. Inhibition of sporulation by alpha-picolinic acid.
J Bacteriol. 1960 Jan;79:1-8 PMID: 13850820
The effect of gluconate in promoting sporulation in Bacillus cereus.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.95.2.355-359.1968

Published

1968-02-00

Pages

355-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC252026

Subset

External Links

PubMed source DOI: 10.1128/jb.95.2.355-359.1968 PMC Full Text View journal details

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Microcycle sporogenesis of Bacillus cereus in a chemically defined medium.

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