Electrophoretic characteristics of staphylococcal hyaluronate lyase.

Journal of bacteriology ·Vol. 97 ·No. 2 ·1969-02-00 ·Pages 715-8

Abramson C, Friedman H

Abstract

Partially purified staphylococcal hyaluronidase was studied with respect to its electrophoretic properties by the use of starch slurry or semisolid Noble agar as a matrix. The polarity of enzyme activity was found to be cathodal on starch and anodal on agar gel. The electrophoretic migration of the partially purified enzyme on starch, as a function of pH, suggested that the enzyme has an isoelectric point of pH 9.5 to 10.

MeSH Terms

Electrophoresis Hyaluronoglucosaminidase/analysis Hydrogen-Ion Concentration Immunodiffusion Staphylococcus/enzymology

Chemicals

Hyaluronoglucosaminidase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Abramson C

Friedman H

References (9)

9 references, click to expand

Adsorption of proteins on 'Sephadex'.
Nature. 1962 Jun 2;194:862-3 PMID: 13899167
Zone electrophoresis in a starch supporting medium.
Proc Soc Exp Biol Med. 1952 May;80(1):42-4 PMID: 14930066
Studies on extracellular PROTEINS FROM Staphylococcus aureus. I. Separation and characterization of enzymes and toxins by isoelectric focusing.
Biochim Biophys Acta. 1967 Apr 11;133(3):435-45 PMID: 6033794
Agarose as an anticonvection agent in zone electrophoresis.
Biochim Biophys Acta. 1961 Nov 11;53:514-7 PMID: 13907812
Staphylococcal hyaluronate lyase: multiple electrophoretic and chromatographic forms.
Arch Biochem Biophys. 1967 Jul;121(1):103-6 PMID: 4962288
Staphylococcal hyaluronate lyase: purification and characterization studies.
J Bacteriol. 1968 Oct;96(4):886-92 PMID: 4301047
Studies on diffusing factors: 2. Methods of assay of hyaluronidase and their correlation with skin diffusing activity.
Biochem J. 1943 Jul;37(2):169-77 PMID: 16747611
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
FACTORS INFLUENCING THE ELECTROPHORETIC MIGRATION OF LACTIC DEHYDROGENASE ISOZYMES.
J Lab Clin Med. 1963 Oct;62:571-8 PMID: 14080856

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.97.2.715-718.1969

Published

1969-02-00

Pages

715-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC249750

Subset

External Links

PubMed source DOI: 10.1128/jb.97.2.715-718.1969 PMC Full Text View journal details

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Electrophoretic characteristics of staphylococcal hyaluronate lyase.

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