Enzymatic cleabage and repair of transforming DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 62 ·No. 1 ·1969-01-00 ·Pages 289-96

Laipis PJ, Olivera BM, Ganesan AT

Abstract

Single-strand nicks caused by DNase I are capable of inactivating B. subtilis transforming DNA. Under suitable conditions, the polynucleotide joining enzyme from E. coli and a similar DPN-requiring activity from B. subtilis can completely repair this damage, restore biological activity, and increase the single-strand molecular weight. The rates of inactivation of a single genetic marker and of a four-marker linkage group suggest that a single-strand nick is inactivating even when far from the site of genetic damage.

MeSH Terms

Bacillus subtilis DNA, Bacterial Deoxyribonucleases Escherichia coli Ligases Transformation, Genetic Tritium

Chemicals

DNA, Bacterial Tritium Deoxyribonucleases Ligases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Laipis P J

Olivera B M

Ganesan A T

References (13)

13 references, click to expand

Integration of deoxyribonuclease-treated DNA in bacillus subtilis transformation.
J Gen Physiol. 1966 Jul;49(6):233-58 PMID: 4961186
Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage.
Proc Natl Acad Sci U S A. 1967 Apr;57(4):1021-8 PMID: 5340583
Diphosphopyridine nucleotide: a cofactor for the polynucleotide-joining enzyme from Escherichia coli.
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1700-4 PMID: 4291945
Enzymatic joining of DNA strands: a novel reaction of diphosphopyridine nucleotide.
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1841-8 PMID: 4291949
Linkage of polynucleotides through phosphodiester bonds by an enzyme from Escherichia coli.
Proc Natl Acad Sci U S A. 1967 May;57(5):1426-33 PMID: 5341238
A biological assay for polynucleotide ligase: recovery of marker activity in DNA-transformation.
Biochem Biophys Res Commun. 1967 Aug 23;28(4):641-6 PMID: 4861160
The enzymatic repair of DNA, II. Characterization of phage-induced sealase.
Proc Natl Acad Sci U S A. 1967 Nov;58(5):1996-2003 PMID: 4295584
Repair of single strand breaks in transforming DNA by polynucleotide ligase.
Biochem Biophys Res Commun. 1968 May 23;31(4):540-4 PMID: 4968521
Sedimentation rate as a measure of molecular weight of DNA.
Biophys J. 1963 Jul;3:309-21 PMID: 14016998
ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.
J Biol Chem. 1964 Jan;239:222-32 PMID: 14114848
A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. I. PURIFICATION OF THE ENZYME AND CHARACTERIZATION OF THE PHOSPHATASE ACTIVITY.
J Biol Chem. 1964 Jan;239:242-50 PMID: 14114850
PHYSICAL AND BIOLOGICAL STUDIES ON TRANSFORMING DNA.
J Mol Biol. 1964 Sep;9:683-95 PMID: 14216611
BIOCHEMICAL AND GENETIC STUDIES OF INTEGRATION AND RECOMBINATION IN BACILLUS SUBTILIS TRANSFORMATION.
Genetics. 1964 Oct;50:717-38 PMID: 14221877

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.62.1.289

Published

1969-01-00

Pages

289-96

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC285986

Subset

External Links

PubMed source DOI: 10.1073/pnas.62.1.289 PMC Full Text View journal details

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