Effect of lysozyme on resting spores of Bacillus megaterium.

Journal of bacteriology ·Vol. 98 ·No. 1 ·1969-04-00 ·Pages 238-45

Suzuki Y, Rode LJ

Abstract

Resting spores of Bacillus megaterium ATCC 9885 were found to be markedly affected by lysozyme. Exposure to as little as 1.5 mug of lysozyme per ml caused the spores to lose refractility, the darkened spores to shed their coat structures, and the spore central bodies to lyse. The spores of seven other strains of B. megaterium and seven other Bacillus species were not similarly affected by lysozyme. Proteolytic enzymes such as pronase, trypsin, pepsin, and subtilisin did not induce the change. The action of lysozyme differed in certain important respects from that of common "physiological" germinants. Its action was considered to be direct via its enzymatic attack on exposed sites directly accessible in the resting spores of B. megaterium ATCC 9885.

MeSH Terms

Alanine/pharmacology Alkanes/pharmacology Bacillus/drug effects Bacillus megaterium/drug effects Bacteriolysis Enzymes/pharmacology Glucose/pharmacology Leucine/pharmacology Microscopy, Electron Muramidase/pharmacology Nucleosides/pharmacology Spores/drug effects,growth & development

Chemicals

Alkanes Enzymes Nucleosides Muramidase Leucine Glucose Alanine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Suzuki Y

Rode L J

References (32)

32 references, click to expand

Influence of sugars and amines on the colorimetric hexosamine method of elson and morgan and its possible elimination.
Nature. 1950 Jun 3;165(4205):898 PMID: 15423524
Utility of sodium hypochlorite for ultrastructure study of bacterial spore integuments.
J Bacteriol. 1966 Dec;92(6):1772-8 PMID: 4959721
Fine structure of Bacillus megaterium during microcycle sporogenesis.
J Bacteriol. 1967 Aug;94(2):441-57 PMID: 4962705
Response of Bacillus spores to combinations of germinative compounds.
J Bacteriol. 1966 Mar;91(3):1168-77 PMID: 4956331
GERMINATION OF BACTERIAL SPORES BY SUBTILISIN.
Can J Microbiol. 1964 Dec;10:929-31 PMID: 14272493
Base exchange and heat resistance in bacterial spores.
Biochem Biophys Res Commun. 1963 Jan 31;10:139-43 PMID: 14011814
HEAT ADAPTATION AND ION EXCHANGE IN BACILLUS MEGATERIUM SPORES.
Science. 1964 Jan 10;143(3602):141-3 PMID: 14075725
Chemical factors in the germination of spore-bearing aerobes; the effect of yeast extract on the germination of Bacillus anthracis and its replacement by adenosine.
Biochem J. 1949;45(3):353-62 PMID: 15392863
Changes in spores of Bacillus megaterium treated with thioglycolate at a low pH and restoration of germinability and heat resistance by cations.
J Bacteriol. 1967 Mar;93(3):1017-22 PMID: 4960916
Chemical factors in the germination of spore-bearing aerobes; the effects of amino acids on the germination of Bacillus anthracis, with some observations on the relation of optical form to biological activity.
Biochem J. 1949;45(3):363-70 PMID: 15392864
FLUOROCHROME-LABELLED LYSOZYME: REAGENT FOR THE DETECTION OF LYSOZYME SUBSTRATE IN CELLS.
Nature. 1963 Oct 26;200:385-6 PMID: 14087911
Reduced lysis at high concentrations of lysozyme.
Arch Biochem Biophys. 1954 Oct;52(2):305-12 PMID: 13208254
The composition of the spore coats of Bacillus megatherium, B. subtilis and B. cereus.
Biochem J. 1956 Mar;62(3):459-65 PMID: 13303998
Correlation between spore structure and spore properties in Bacillus megaterium.
J Bacteriol. 1968 Jun;95(6):1979-86 PMID: 4970217
Colorimetric assay for dipicolinic acid in bacterial spores.
Science. 1958 Jan 3;127(3288):26-7 PMID: 13495474
Ionic germination of spores of Bacillus megaterium QM B 1551.
Arch Mikrobiol. 1962;43:183-200 PMID: 14038736
SPORULATION OF PROTOPLASTS.
Folia Microbiol (Praha). 1964 Jan;23:21-3 PMID: 14117875
Factors affecting the germination of thick suspensions of bacillus subtilis spores in L-alanine solution.
J Gen Microbiol. 1950 Sep;4(3):330-8 PMID: 14778937
Germination of bacterial endospores with subtilopeptidases.
Can J Microbiol. 1967 May;13(5):489-501 PMID: 4962685
Hexosamine-containing peptides in spores of Bacillus subtilis, B. megatherium and B. cereus.
Biochem J. 1954 Sep;58(1):80-5 PMID: 13198854
Influence of exchangeable ions on germinability of bacterial spores.
J Bacteriol. 1966 Apr;91(4):1582-8 PMID: 4956343
Location and composition of spore mucopeptide in Bacillus species.
J Cell Biol. 1963 Mar;16:593-609 PMID: 13999017
SENSITIZATION OF BACTERIAL SPORES TO LYSOZYME AND TO HYDROGEN PEROXIDE WITH AGENTS WHICH RUPTURE DISULPHIDE BONDS.
J Gen Microbiol. 1963 Dec;33:413-23 PMID: 14114930
GASEOUS HYDROCARBONS AND THE GERMINATION OF BACTERIAL SPORES.
Proc Natl Acad Sci U S A. 1965 Jan;53:31-8 PMID: 14283204
MECHANICAL GERMINATION OF BACTERIAL SPORES.
Proc Natl Acad Sci U S A. 1960 Jan;46(1):118-28 PMID: 16590585
Germination of bacterial spores with alkyl primary amines.
J Bacteriol. 1961 May;81:768-79 PMID: 13742511
A cell-wall lytic enzyme associated with spores of Bacillus species.
J Gen Microbiol. 1957 Feb;16(1):236-49 PMID: 13406233
Germination of bacterial endospores with calcium and dipicolinic acid.
Science. 1961 May 26;133(3465):1703-4 PMID: 13741539
Initiation of bacterial spore germination.
J Bacteriol. 1968 Apr;95(4):1327-34 PMID: 4296513
Role of amino-acids in the germination of bacterial spores by subtilisn.
Nature. 1966 Sep 24;211(5056):1431-2 PMID: 4961424
Biochemical changes occurring during the germination of bacterial spores.
Biochem J. 1953 May;54(2):205-9 PMID: 13058859
A colorimetric method for the determination of glucosamine and chondrosamine.
Biochem J. 1933;27(6):1824-8 PMID: 16745305

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.98.1.238-245.1969

Published

1969-04-00

Pages

238-45

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC249929

Subset

External Links

PubMed source DOI: 10.1128/jb.98.1.238-245.1969 PMC Full Text View journal details

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Effect of lysozyme on resting spores of Bacillus megaterium.

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