Home LiteratureArticle Details
PMID: 4979441 Published · ppublish English Journal Article

Characterization of the deoxyribonucleic acid of various strains of halophilic bacteria.

Journal of bacteriology ·Vol. 99 ·No. 1 ·1969-07-00 ·Pages 248-54

Moore RL, McCarthy BJ

Abstract

Bacteria classified as extreme halophiles, in the genera Halobacterium and Halococcus, contain deoxyribonucleic acid (DNA) which displays two components in a CsCl equilibrium density gradient. The base composition of the major DNA component ranges from 66 to 68% guanine plus cytosine (GC), whereas that of the satellite DNA comprising some 11 to 36% of the total, is between 57 and 60% GC. Purification of the bacterial cells in a CsCl density gradient and other more conventional strain purification procedures both indicated that the presence of the satellite DNA component is not a result of mixed cultures.

MeSH Terms
Acridines/pharmacology Bacteria/analysis,drug effects,metabolism Centrifugation, Density Gradient DNA, Bacterial/analysis Escherichia coli/analysis Halobacterium/analysis Mitomycins/pharmacology Pseudomonas aeruginosa/analysis Rhodopseudomonas/analysis Sodium Chloride/metabolism
Chemicals
Acridines DNA, Bacterial Mitomycins Sodium Chloride
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Moore R L
McCarthy B J
References (37)
37 references, click to expand
  1. The glycerol dehydrogenases of Pseudomonas salinaria, Vibrio costicolus, and Escherichia coli in relation to bacterial halophilism.
    Can J Biochem Physiol. 1954 May;32(3):206-17 PMID: 13150244
  2. The effect of chlorides of monovalent cations, urea, detergents, and heat on morphology and the turbidity of suspensions of red halophilic bacteria.
    Can J Microbiol. 1961 Oct;7:741-50 PMID: 13859031
  3. Base sequence homology and renaturation studies of the deoxyribonucleic acid of extremely halophilic bacteria.
    J Bacteriol. 1969 Jul;99(1):255-62 PMID: 5802609
  4. High-Resolution Density Gradient Sedimentation Analysis.
    Science. 1960 Jan 1;131(3392):32-3 PMID: 17794662
  5. LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER.
    Proc Natl Acad Sci U S A. 1965 Apr;53:737-45 PMID: 14324529
  6. Selective inhibition of formation of deoxyribonucleic acid in Escherichia coli by mitomycin C.
    Nature. 1959 Apr 11;183(4667):1056-7 PMID: 13644283
  7. Zone centrifugation in a cesium chloride density gradient caused by temperature change.
    Biophys J. 1965 Nov;5(6):947-64 PMID: 5884018
  8. SOME PROPERTIES OF NUCLEIC ACIDS EXTRACTED WITH PHENOL.
    Biochim Biophys Acta. 1965 Feb 8;95:329-40 PMID: 14293707
  9. Solute concentrations within cells of halophilic and non-halophilic bacteria.
    Biochim Biophys Acta. 1962 Dec 17;65:506-8 PMID: 14021131
  10. The effect of magnesium, potassium, and iron on the growth and morphology of red halophilic bacteria.
    Can J Microbiol. 1955 Aug;1(7):486-94 PMID: 13250443
  11. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
    J Mol Biol. 1962 Jun;4:430-43 PMID: 14498379
  12. The freezing points of bacterial cells in relation to halophilism.
    J Gen Microbiol. 1959 Feb;20(1):27-31 PMID: 13631179
  13. The cysteine desulphydrase of Pseudomonas salinaria.
    Can J Microbiol. 1957 Apr;3(3):461-5 PMID: 13437206
  14. NEW APPROACHES TO BACTERIAL TAXONOMY.
    Annu Rev Microbiol. 1963;17:329-72 PMID: 14147455
  15. Structural considerations in the interaction of DNA and acridines.
    J Mol Biol. 1961 Feb;3:18-30 PMID: 13761054
  16. Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients.
    J Bacteriol. 1968 Mar;95(3):867-75 PMID: 4966830
  17. Effects of sodium and potassium chloride on certain enzymes of Micrococcus halodenitrificans and Pseudomonas salinaria.
    Can J Microbiol. 1956 Oct;2(6):599-606 PMID: 13374606
  18. Purification of competent cells in the Bacillus subtilis transformation system.
    J Bacteriol. 1968 Mar;95(3):876-85 PMID: 4966831
  19. EFFECT OF INHIBITORS OF NUCLEIC ACID AND PROTEIN SYNTHESES ON THE INDUCED SYNTHESES OF BACTERIOCHLOROPHYLL AND DELTA-AMINOLEVULINIC ACID SYNTHETASE BY RHODOPSEUDOMONAS SPHEROIDES.
    Biochim Biophys Acta. 1965 Jan 11;95:94-110 PMID: 14289040
  20. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI.
    Proc Natl Acad Sci U S A. 1960 Jan;46(1):57-64 PMID: 16590598
  21. Deoxyribonucleic Acid of the blue-green algae (cyanophyta).
    Bacteriol Rev. 1967 Dec;31(4):315-31 PMID: 16350207
  22. An index to deoxyribonucleic acid base compositions of bacterial species.
    J Gen Microbiol. 1966 Sep;44(3):419-37 PMID: 5339419
  23. The isolation and preliminary characterization of a halophilic photosynthetic bacterium.
    Arch Mikrobiol. 1967;59(1):255-68 PMID: 4880241
  24. THE RIBOSOMES OF THE EXTREMELY HALOPHILIC BACTERIUM, HALOBACTERIUM CUTIRUBRUM.
    J Mol Biol. 1964 Sep;9:654-69 PMID: 14216609
  25. Comparative study of ribosomal ribonucleic acid cistrons in enterobacteria and myxobacteria.
    J Bacteriol. 1967 Oct;94(4):1066-74 PMID: 4860906
  26. The synthesis and function of ribosomes in a new mutant of Escherichia coli.
    Proc Natl Acad Sci U S A. 1967 Jan;57(1):141-7 PMID: 4860191
  27. Dependence of the density of deoxyribonucleic acids on guanine-cytosine content.
    Nature. 1959 May 23;183(4673):1429-31 PMID: 13657153
  28. Satellite DNA in photosynthetic bacteria.
    Biochem Biophys Res Commun. 1966 Aug 23;24(4):549-53 PMID: 5967242
  29. Elimination of transmissible drug-resistance by treatment with acriflavin.
    Nature. 1961 Mar 18;189:947 PMID: 13771419
  30. The presence of two species of DNA in some halobacteria.
    J Mol Biol. 1963 Jan;6:34-8 PMID: 13964964
  31. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.
    J Mol Biol. 1962 Jul;5:109-18 PMID: 14470099
  32. A cell-free amino acid incorporating system from an extremely halophilic bacterium.
    Biochemistry. 1968 Jun;7(6):2249-56 PMID: 5660049
  33. A MOLECULAR MECHANISM OF MITOMYCIN ACTION: LINKING OF COMPLEMENTARY DNA STRANDS.
    Proc Natl Acad Sci U S A. 1963 Aug;50:355-62 PMID: 14060656
  34. RHODOMICROBIUM VANNIELII, A NEW PHOTOHETEROTROPHIC BACTERIUM.
    J Bacteriol. 1949 Oct;58(4):409-16 PMID: 16561801
  35. A morphological study of Halobacterium halobium and its lysis in media of low salt concentration.
    J Cell Biol. 1967 Jul;34(1):365-93 PMID: 6033542
  36. Turbidity of suspensions and morphology of red halophilic bacteria as influenced by sodium chloride concentration.
    Can J Microbiol. 1960 Oct;6:535-43 PMID: 13681109
  37. Heterogeneity in deoxyribonucleic acids. I. Dependence on composition of the configurational stability of deoxyribonucleic acids.
    Nature. 1959 May 23;183(4673):1427-9 PMID: 13657152
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.99.1.248-254.1969
Published
1969-07-00
Pages
248-54
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC249995
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.99.1.248-254.1969 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2