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PMID: 4982359 Published · ppublish English Journal Article

Ribosomal RNA genes in bacteria: evidence for the nature of the physical linkage between 16S and 23S RNA genes in Bacillus subtilis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 64 ·No. 2 ·1969-10-00 ·Pages 642-9

Colli W, Oishi M

Abstract

The nature of the arrangement of 16S and 23S ribosomal RNA genes on the Bacillus subtilis chromosome was studied by means of a Cs(2)SO(4) density gradient centrifugation technique after complexing DNA-(ribosomal) RNA hybrids with mercuric ions. It was observed that the same fragments of single-stranded DNA with an average molecular weight of 1.9 x 10(6) which hybridize with 23S RNA also hybridize with 16S RNA. This indicates that genes for both these species of ribosomal RNA are physically linked on such DNA fragments. Some evidence suggests that the two types of genes are interspersed with each other, perhaps in sets, each of which contains a 16S and a 23S ribosomal RNA gene.

MeSH Terms
Bacillus subtilis Centrifugation, Density Gradient Chromatography DNA, Bacterial Genetics, Microbial Hybridization, Genetic Molecular Weight RNA, Bacterial Ribosomes
Chemicals
DNA, Bacterial RNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Colli W
Oishi M
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.64.2.642
Published
1969-10-00
Pages
642-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC223392
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.64.2.642 PMC Full Text View journal details
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