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PMID: 4982890 Published · ppublish English Journal Article

Isozymes of beta-glucosidase in Dictyostelium discoideum.

Journal of bacteriology ·Vol. 100 ·No. 3 ·1969-12-00 ·Pages 1208-17

Coston MB, Loomis WF

Abstract

The activity of beta-glucosidase (EC 3.2.1.21) in extracts of Dictyostelium discoideum was investigated. The specific activity increased early in development, declined during pseudoplasmodium formation, and increased again during sorocarp formation. The beta-glucosidase which was present in growing amoebae and during the first stages of multicellular development was electrophoretically distinct from the enzyme which accumulated during the final stages of morphogenesis. Ribonucleic acid synthesis and protein synthesis during development were required for the accumulation of the later isozyme. Analysis of beta-glucosidase activity in a number of morphological mutants suggests that the enzyme which accumulates late in morphogenesis is developmentally controlled.

MeSH Terms
Centrifugation, Density Gradient Chromatography, Thin Layer Cycloheximide/pharmacology Dactinomycin/pharmacology Edetic Acid/pharmacology Electrophoresis, Disc Glucosidases/isolation & purification,metabolism Isoenzymes Mutation Myxomycetes/drug effects,enzymology,growth & development Protein Biosynthesis RNA/biosynthesis
Chemicals
Isoenzymes Dactinomycin RNA Cycloheximide Edetic Acid Glucosidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Coston M B
Loomis W F
References (20)
20 references, click to expand
  1. The regulatory program for UDPgalactose polysaccharide transferase activity during slime mold cytodifferentiation: requirement for specific synthesis of ribonucleic acid.
    Biochim Biophys Acta. 1965 Nov 8;108(3):463-73 PMID: 5867535
  2. The appearance and disappearance of uridine diphosphate glucose pyrophosphorylase activity during differntiation of the cellular slime mold Dictyostelium discoideum.
    J Biol Chem. 1967 Apr 25;242(8):1696-700 PMID: 6067273
  3. Developmental regulation of the enzyme UDP-galactose polysaccharide transferase.
    Exp Cell Res. 1967 May;46(2):328-34 PMID: 4226096
  4. Trehalose synthesis in the cellular slime mold Dictyostelium discoideum.
    Biochim Biophys Acta. 1966 Aug 10;122(2):225-31 PMID: 6007986
  5. Commitment to the synthesis of a specific enzyme during cellular slime mold development.
    J Mol Biol. 1966 Dec 28;22(2):401-4 PMID: 4961730
  6. The effect of actinomycin D on cellular slime mold morphogenesis.
    Biochem Biophys Res Commun. 1967 Feb 8;26(3):353-9 PMID: 6068065
  7. Periods of genetic transcription required for the synthesis of three enzymes during cellular slime mold development.
    Proc Natl Acad Sci U S A. 1968 Apr;59(4):1235-42 PMID: 5240026
  8. Trehalose 6-phosphate synthetase (uridine diphosphate glucose: d-glucose 6-phosphate 1-glucosyltransferase) and its regulation during slime mold development.
    J Biol Chem. 1968 Oct 10;243(19):5081-7 PMID: 5692877
  9. The relation between cytodifferentiation and inactivation of a developmentally controlled enzyme in Dictyostelium discoideum.
    Exp Cell Res. 1968 Oct;53(1):283-7 PMID: 5749523
  10. Acetylglucosaminidase, an early enzyme in the development of Dictyostelium discoideum.
    J Bacteriol. 1969 Mar;97(3):1149-54 PMID: 5813341
  11. Cellulolytic enzymes during morphogenesis in Dictyostelium discoideum.
    J Bacteriol. 1968 Sep;96(3):639-45 PMID: 5753557
  12. Detection of sugars on paper chromatograms.
    Nature. 1950 Sep 9;166(4219):444-5 PMID: 14775715
  13. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  14. [Cell functions and change in cell function in the development of Dictyostelium discoideum. V. Stagespecific cell contact formation and its quantitative evaluation].
    Exp Cell Res. 1961 Dec;25:535-54 PMID: 13897956
  15. A mutation affecting both rate and pattern of morphogenesis in Dictyostelium discoideum.
    Dev Biol. 1963 Mar;6:79-93 PMID: 13978227
  16. Metabolism of major cell components during slime mold morphogenesis.
    Biochim Biophys Acta. 1961 Oct 28;53:285-93 PMID: 14006519
  17. UDP-GALACTOSE POLYSACCHARIDE TRANSFERASE IN THE CELLULAR SLIME MOLD, DICTYOSTELIUM DISCOIDEUM: APPEARANCE AND DISAPPEARANCE OF ACTIVITY DURING CELL DIFFERENTIATION.
    Proc Natl Acad Sci U S A. 1964 Jul;52:81-7 PMID: 14192661
  18. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
    Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
  19. [ROLE OF LACTOSE AND ITS METABOLIC PRODUCTS IN THE INDUCTION OF THE LACTOSE OPERON IN ESCHERICHIA COLI].
    Biochim Biophys Acta. 1965 Apr 19;95:634-9 PMID: 14324815
  20. [Studies on lactic acid splitting enzyme. III. Purification, crystallization and features of beta-galctosidase of Escherichia coli ML 309].
    Biochem Z. 1959;331:459-85 PMID: 13842616
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.100.3.1208-1217.1969
Published
1969-12-00
Pages
1208-17
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC250295
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.100.3.1208-1217.1969 PMC Full Text View journal details
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