Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation.

Applied microbiology ·Vol. 18 ·No. 5 ·1969-11-00 ·Pages 824-33

Hierholzer JC, Suggs MT, Hall EC

Abstract

Standardized hemagglutination and hemagglutination-inhibition procedures are described and statistically evaluated for all animal viruses where applicable, except for rubella and the arbovirus group. The standardized tests employ a constant phosphate-buffered saline diluent and constant volumes of serum, antigen, and standardized erythrocyte suspension. The standardized hemagglutination test has a reproducibility of 84 to 96% with adenoviruses, rubeola, and the myxoviruses, and 78 to 93% with reoviruses; the standardized hemagglutination-inhibition test has a reproducibility of 95 to 100% with all viruses tested.

MeSH Terms

Adenoviridae/immunology Antigens Buffers Erythrocytes Hemagglutination Inhibition Tests/standards Hemagglutination Tests/standards Immune Sera Orthomyxoviridae/immunology Rubella virus/immunology Statistics as Topic Viruses/immunology

Chemicals

Antigens Buffers Immune Sera

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hierholzer J C

Suggs M T

Hall E C

References (18)

18 references, click to expand

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Standardized viral hemagglutination and hemagglutination-inhibition tests. I. Standardization of erythrocyte suspensions.
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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.18.5.824-833.1969

Published

1969-11-00

Pages

824-33

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC378095

Subset

External Links

PubMed source DOI: 10.1128/am.18.5.824-833.1969 PMC Full Text

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