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PMID: 4984237 Published · ppublish English Journal Article

A specific complex between a restriction endonuclease and its DNA substrate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 65 ·No. 2 ·1970-02-00 ·Pages 357-62

Yuan R, Meselson M

Abstract

In the presence of Mg(++), ATP, and S-adenosylmethionine, the DNA restriction endonuclease R.K forms a specific complex with its DNA substrate. The complex can be detected by its retention on nitrocellulose membranes.

MeSH Terms
Adenosine Triphosphate Cellulose Chemical Phenomena Chemistry Coliphages DNA, Viral Deoxyribonucleases Edetic Acid Escherichia coli/enzymology Kinetics Magnesium Membranes, Artificial Methionine Nitrates Nucleosides
Chemicals
DNA, Viral Membranes, Artificial Nitrates Nucleosides Adenosine Triphosphate Cellulose Edetic Acid Methionine Deoxyribonucleases Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yuan R
Meselson M
References (7)
7 references, click to expand
  1. Studies on the binding of RNA polymerase to polynucleotides.
    J Mol Biol. 1966 Dec 28;22(2):199-209 PMID: 5972769
  2. Recognition of tRNA by aminoacyl tRNA synthetases.
    J Mol Biol. 1967 Sep 28;28(3):479-90 PMID: 4861180
  3. DNA restriction enzyme from E. coli.
    Nature. 1968 Mar 23;217(5134):1110-4 PMID: 4868368
  4. Host specificity of DNA produced by Escherichia coli, X. In vitro restriction of phage fd replicative form.
    Proc Natl Acad Sci U S A. 1968 Apr;59(4):1300-6 PMID: 4870862
  5. DNA modification and restriction.
    Annu Rev Biochem. 1969;38:467-500 PMID: 4897066
  6. The Escherichia coli B restriction endonuclease.
    Biochim Biophys Acta. 1969 Nov 19;195(1):219-29 PMID: 4901831
  7. DNA binding of the lac repressor.
    J Mol Biol. 1968 Jul 14;34(2):365-8 PMID: 4938552
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.65.2.357
Published
1970-02-00
Pages
357-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282910
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.65.2.357 PMC Full Text View journal details
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