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PMID: 4988689 Published · ppublish English Journal Article

The effect of ethylenediaminetetra-acetate on Pseudomonas alcaligenes and the composition of the bacterial cell wall.

The Biochemical journal ·Vol. 117 ·No. 4 ·1970-05-00 ·Pages 721-32

Key BA, Gray GW, Wilkinson SG

Abstract

1. EDTA in borate buffer has a marked bactericidal effect on Pseudomonas alcaligenes, which is more sensitive than Pseudomonas aeruginosa. The bactericidal effect is accompanied by solubilization of lipopolysaccharide and release of intracellular solutes. These effects are more pronounced at pH9.2 than 7.1. 2. Cell walls of P. alcaligenes were prepared and from them were obtained the readily extracted lipids and the fractions given by treatment with aqueous phenol. 3. The cell walls and the above components were analysed and results are compared with those for P. aeruginosa. 4. Lipopolysaccharide obtained by treatment of cell walls with aqueous phenol is contaminated with glycosaminopeptide to a variable extent. 5. The lipopolysaccharide contains less neutral sugar but more phosphorus than the lipopolysaccharide of P. aeruginosa; fucosamine is not a component of the lipopolysaccharide of P. alcaligenes.

MeSH Terms
Amino Acids/analysis Cell Wall/analysis,drug effects Chromatography, Gas DNA, Bacterial/analysis Edetic Acid/pharmacology Fatty Acids/analysis Hexoses/analysis Hydrogen-Ion Concentration Lipopolysaccharides/analysis Phosphorus/analysis Pseudomonas/drug effects Pseudomonas aeruginosa/analysis Ribose/analysis
Chemicals
Amino Acids DNA, Bacterial Fatty Acids Hexoses Lipopolysaccharides Phosphorus Ribose Edetic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Key B A
Gray G W
Wilkinson S G
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40 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1170721
Published
1970-05-00
Pages
721-32
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1179024
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1170721 PMC Full Text View journal details
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