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PMID: 4990876 Published · ppublish English Journal Article

Subunit heterogeneity in human serum beta lipoprotein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 66 ·No. 4 ·1970-08-00 ·Pages 1075-82

Kane JP, Richards EG, Havel RJ

Abstract

The apoprotein of human serum beta lipoprotein has been prepared in a lipid-free and soluble form by a technique of interfacial extraction in the presence of guanidinium hydrochloride and nonionic amphiphile. Gel permeation chromatography of maleylated apobeta lipoprotein separates two protein fractions with different amino acid compositions. The smaller particle has a molecular weight of 2.6 x 10(4).

MeSH Terms
Amino Acids/analysis Chromatography, Gel Chromatography, Ion Exchange Guanidines Humans Immunodiffusion Lipoproteins/isolation & purification Male
Chemicals
Amino Acids Guanidines Lipoproteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kane J P
Richards E G
Havel R J
References (23)
23 references, click to expand
  1. Chromatography of myosin on diethylaminoethyl-Sephadex A-50.
    Biochemistry. 1967 Feb;6(2):528-40 PMID: 6047636
  2. A revision of the Schoenheimer-Sperry method for cholesterol determination.
    J Biol Chem. 1950 Nov;187(1):97-106 PMID: 14794694
  3. Studies of the proteins in human plasma very low density lipoproteins.
    J Biol Chem. 1969 Oct 25;244(20):5687-94 PMID: 4981584
  4. Heterogeneity of maleylated and partially delipidated low density lipoproteins from human plasma.
    Ann Med Exp Biol Fenn. 1969;47(1):48-51 PMID: 4983181
  5. On the geometrical arrangement of the protein subunits of human serum low-density lipoprotein: evidence for a dodecahedral model.
    Proc Natl Acad Sci U S A. 1969 Sep;64(1):304-10 PMID: 5263015
  6. Some methods of electron microscopic visualization of lipoproteins in plasma and chyle.
    J Histochem Cytochem. 1968 May;16(5):366-70 PMID: 5656147
  7. The protein moiety of human serum beta-lipoproteins.
    Biochem Biophys Res Commun. 1967 Sep 27;28(6):1003-7 PMID: 6064578
  8. Some physical and chemical studies on two polypeptide components of high-density lipoproteins of human serum.
    Biochemistry. 1968 Oct;7(10):3396-403 PMID: 5681453
  9. Isolation and characterization of polypeptides of human serum lipoproteins.
    Biochemistry. 1969 Nov;8(11):4510-6 PMID: 5353112
  10. Electrophoretic separation of plasma lipoproteins in agarose gel.
    J Lipid Res. 1968 Nov;9(6):693-700 PMID: 4176473
  11. THERMODYNAMIC ANALYSIS OF MULTICOMPONENT SOLUTIONS.
    Adv Protein Chem. 1964;19:287-395 PMID: 14268786
  12. Determination of the molecular weight of apoprotein subunits from low density lipoprotein by gel filtration.
    J Lipid Res. 1968 Nov;9(6):789-93 PMID: 5685271
  13. Solubilization and properties of the apoproteins of the very low- and low-density lipoproteins of human serum.
    Biochemistry. 1966 Oct;5(10):3301-8 PMID: 4961658
  14. DETERMINATION OF SERUM TRIGLYCERIDES.
    J Atheroscler Res. 1963 Jul-Aug;3:334-6 PMID: 14051799
  15. Properties of human serum low density lipoproteins after modification by succinic anhydride.
    J Lipid Res. 1968 May;9(3):342-9 PMID: 5650928
  16. Sharper separation of lipoprotein species by paper electrophoresis in albumin-containing buffer.
    J Lab Clin Med. 1963 Mar;61:518-28 PMID: 13929203
  17. Reversible blocking of peptide amino groups by maleic anhydride.
    Biochem J. 1967 Jun;103(3):78P-79P PMID: 6049401
  18. The estimation of polypeptide chain molecular weights by gel filtration in 6 M guanidine hydrochloride.
    J Biol Chem. 1969 Sep 25;244(18):4989-94 PMID: 5824574
  19. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  20. Lipid transport in liver. I. Electron microscopic identification of very low density lipoproteins in perfused rat liver.
    Lab Invest. 1967 Feb;16(2):305-19 PMID: 6023786
  21. The phospholipins of blood.
    Biochem J. 1935 Jul;29(7):1683-9 PMID: 16745836
  22. Studies on the subunit structure of myosin.
    J Biol Chem. 1970 Jan 10;245(1):15-22 PMID: 4243811
  23. Separation and characterization of the protein moiety of human alpha1-lipoprotein.
    Arch Biochem Biophys. 1958 Apr;74(2):390-7 PMID: 13534668
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.66.4.1075
Published
1970-08-00
Pages
1075-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC335788
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.66.4.1075 PMC Full Text View journal details
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