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PMID: 4992015 Published · ppublish English Journal Article

6-(p-hydroxyphenylazo)-uracil: a selective inhibitor of host DNA replication in phage-infected Bacillus subtilis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 3 ·1970-11-00 ·Pages 1454-61

Brown NC

Abstract

The azopyrimidine, 6-(p-hydroxyphenylazo)-uracil, inhibits the replication of bacterial DNA selectively, completely, and reversibly, and has no apparent effects on the metabolism of other cellular macromolecules thus far examined. The mechanism of its action has been investigated in uninfected and phage-infected Bacillus subtilis, and the compound appears to be specific for a host function. In cells infected with virulent phage the synthesis of phage DNA proceeds normally, while residual host DNA synthesis is completely blocked. The drug-sensitive host site retains its sensitivity even after partial disruption of the cell by lysozyme treatment.

MeSH Terms
Azo Compounds/pharmacology Bacillus subtilis/drug effects Bacteriophages Binding Sites DNA Replication/drug effects DNA, Bacterial/antagonists & inhibitors DNA, Viral Phenols/pharmacology Uracil/pharmacology
Chemicals
Azo Compounds DNA, Bacterial DNA, Viral Phenols Uracil
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Brown N C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.67.3.1454
Published
1970-11-00
Pages
1454-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC283373
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.67.3.1454 PMC Full Text View journal details
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