Gene dosage for 5S ribosomal ribonucleic acid in Escherichia coli ad Bacillus megaterium.

Journal of bacteriology ·Vol. 105 ·No. 1 ·1971-01-00 ·Pages 142-9

Pace B, Pace NR

Abstract

The number of gene copies for 5S ribosomal ribonucleic acid (rRNA), relative to that for 16 and 23S rRNA, has been determined by deoxyribonucleic acid (DNA)-RNA hybridization for Escherichia coli and Bacillus megaterium. In both cases, the number of 5S rRNA genes equals the number of 16 or 23S rRNA genes. Rapid procedures for preparing extremely highly purified DNA suitable for DNA-RNA hybridization experiments and chemically pure 5S rRNA are described.

MeSH Terms

Acrylates Autoradiography Bacillus megaterium Centrifugation, Density Gradient Chromatography, DEAE-Cellulose DNA, Bacterial/isolation & purification Ethanol

Chemicals

Acrylates DNA, Bacterial Ethanol

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Pace B

Pace N R

References (12)

12 references, click to expand

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J Bacteriol. 1969 Aug;99(2):535-43 PMID: 4980067
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Proc Natl Acad Sci U S A. 1963 Apr;49:538-44 PMID: 14002462
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Nature. 1970 Jan 3;225(5227):26-9 PMID: 4903099
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Proc Natl Acad Sci U S A. 1970 Apr;65(4):1097-104 PMID: 4909469
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Annu Rev Biochem. 1970;39:183-226 PMID: 4920820
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.105.1.142-149.1971

Published

1971-01-00

Pages

142-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC248333

Subset

External Links

PubMed source DOI: 10.1128/jb.105.1.142-149.1971 PMC Full Text View journal details

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Gene dosage for 5S ribosomal ribonucleic acid in Escherichia coli ad Bacillus megaterium.

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