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PMID: 4993321 Published · ppublish English Journal Article

Sporulation of a cortexless mutant of a variant of Bacillus cereus.

Journal of bacteriology ·Vol. 105 ·No. 1 ·1971-01-00 ·Pages 339-48

Pearce SM, Fitz-James PC

Abstract

A stage 4 sporulation mutant of a strain of Bacillus cereus var. alesti fails to synthesize a cortex although all other structural components appear normal. With terminal lysis the spore core as well as the sporangium is lysed. Both the uptake of (45)Ca and the synthesis of dipicolinic acid (DPA) are similar to these activities in the parent strain, but these components (DPA and Ca) are lost to the medium with the drastic lysis. The first stage of diaminopimelic acid incorporation, that into germ cell wall mucopeptide, is intact in the mutant; the second stage, that into cortical mucopeptide, is absent. These biochemical studies as well as phospholipid metabolism and freeze-etch analysis suggest the lesion lies in the outer forespore membrane.

MeSH Terms
Acetates/metabolism Bacillus cereus/cytology,growth & development,metabolism Calcium Isotopes/metabolism Carbon Isotopes Cell Membrane Cell Wall Chromatography, DEAE-Cellulose Cytoplasmic Granules
Chemicals
Acetates Calcium Isotopes Carbon Isotopes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pearce S M
Fitz-James P C
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.105.1.339-348.1971
Published
1971-01-00
Pages
339-48
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248360
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.105.1.339-348.1971 PMC Full Text View journal details
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