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PMID: 4994028 Published · ppublish English Journal Article

Selection of cells transformed to prototrophy for sporulation markers.

Journal of bacteriology ·Vol. 105 ·No. 3 ·1971-03-00 ·Pages 1200-1

Hoch JA

Abstract

A method is presented for reproducible quantitative selection of recombinants for spore markers in transformation analysis.

MeSH Terms
Acetates Bacillus subtilis/growth & development,isolation & purification Caseins Chloroform Citrates Crosses, Genetic Culture Media DNA, Bacterial Glutamates Lactates Methods Mutation Protein Hydrolysates Recombination, Genetic Spores/growth & development Transformation, Genetic
Chemicals
Acetates Caseins Citrates Culture Media DNA, Bacterial Glutamates Lactates Protein Hydrolysates Chloroform
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Hoch J A
References (5)
5 references, click to expand
  1. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
    J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
  2. [Quantitative study of transformation in sporulation mutants of Bacillus subtilis].
    Ann Inst Pasteur (Paris). 1967 Aug;113(2):157-62 PMID: 4964300
  3. CHEMICALLY DEFINED, SYNTHETIC MEDIA FOR SPORULATION AND FOR GERMINATION AND GROWTH OF BACILLUS SUBTILIS.
    J Bacteriol. 1964 Feb;87:332-6 PMID: 14151053
  4. A study of the genetic material determining an enzyme in Pneumococcus.
    Biochim Biophys Acta. 1960 Apr 22;39:508-18 PMID: 14413322
  5. Genetic analysis of pleiotropic negative sporulation mutants in Bacillus subtilis.
    J Bacteriol. 1971 Mar;105(3):896-901 PMID: 4994039
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1971-03-00
Pages
1200-1
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248557
Subset
IM
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