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PMID: 4994038 Published · ppublish English Journal Article

Mechanism of resistance to antibiotic synergism in enterococci.

Journal of bacteriology ·Vol. 105 ·No. 3 ·1971-03-00 ·Pages 873-9

Zimmermann RA, Moellering RC, Weinberg AN

Abstract

Enterococci exhibit two types of resistance to streptomycin. Moderately high-level resistance is observed in most naturally occurring strains and can be overcome by simultaneous exposure to penicillin. In addition, very high-level resistance is found in those strains against which penicillin plus streptomycin fail to produce synergism in vitro. To study the mechanism of streptomycin resistance in enterococci, ribosomes from a wild-type strain and from a highly streptomycin-resistant mutant were isolated, characterized, and studied in an in vitro amino acid incorporation system. The ribosomes from the organism with moderately high-level streptomycin resistance were sensitive to streptomycin in vitro, suggesting that this type of resistance is caused by failure of streptomycin to reach the ribosomes. Very high-level resistance (and lack of penicillin-streptomycin synergism), on the other hand, appears to be due to ribosomally mediated streptomycin resistance.

MeSH Terms
Amino Acids/metabolism Carbon Isotopes Centrifugation, Density Gradient Dialysis Drug Synergism Enterococcus faecalis/drug effects Genetics, Microbial Magnesium Nucleosides Penicillin Resistance Penicillins/pharmacology Ribosomes/drug effects,metabolism Streptomycin/pharmacology Sucrose
Chemicals
Amino Acids Carbon Isotopes Nucleosides Penicillins Sucrose Magnesium Streptomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zimmermann R A
Moellering R C
Weinberg A N
References (18)
18 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.105.3.873-879.1971
Published
1971-03-00
Pages
873-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248512
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.105.3.873-879.1971 PMC Full Text View journal details
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