Bacterial inactivation of transforming deoxyribonucleate.

Journal of bacteriology ·Vol. 107 ·No. 3 ·1971-09-00 ·Pages 889-99

Haseltine FP, Fox MS

Abstract

Competent and noncompetent bacteria are able to bind deoxyribonucleate (DNA). The consequence of this binding is different for the two types of bacteria. Competent bacteria are able to utilize the DNA for transformation. DNA exposed to noncompetent bacteria loses its biological activity with a coincident reduction in the double-stranded molecular weight. The reduction in molecular weight does not appear to be sufficient to account for the loss of biological activity observed.

MeSH Terms

Bacillus subtilis Carbon Isotopes Centrifugation, Density Gradient DNA, Bacterial/isolation & purification Diatrizoate Genetics, Microbial Molecular Weight Phosphorus Isotopes Ribonucleases Salmonella Phages Sucrose Thymine Transformation, Genetic

Chemicals

Carbon Isotopes DNA, Bacterial Phosphorus Isotopes Diatrizoate Sucrose Ribonucleases Thymine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Haseltine F P

Fox M S

References (11)

11 references, click to expand

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J Bacteriol. 1968 Mar;95(3):867-75 PMID: 4966830
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Sedimentation rate as a measure of molecular weight of DNA.
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Purification of competent cells in the Bacillus subtilis transformation system.
J Bacteriol. 1968 Mar;95(3):876-85 PMID: 4966831
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J Mol Biol. 1965 Feb;11:373-90 PMID: 14290352
Mutations in the temperate phage P22 and lysogeny in Salmonella.
Virology. 1957 Feb;3(1):22-41 PMID: 13409758
Genetic transformation. I. Cellular incorporation of DNA accompanying transformation in Pneumococcus.
Biochim Biophys Acta. 1957 Oct;26(1):68-82 PMID: 13479462
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J Mol Biol. 1968 Jun 28;34(3):621-41 PMID: 4938561
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J Bacteriol. 1969 Mar;97(3):1426-30 PMID: 4975749
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J Mol Biol. 1964 Sep;9:683-95 PMID: 14216611

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.107.3.889-899.1971

Published

1971-09-00

Pages

889-99

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247015

Subset

External Links

PubMed source DOI: 10.1128/jb.107.3.889-899.1971 PMC Full Text View journal details

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