Simultaneous use of contrasting fluorochromes to separate measles and canine distemper viruses in a common system.

Applied microbiology ·Vol. 22 ·No. 3 ·1971-09-00 ·Pages 459-62

Gourlay JA, Pemberton JR

Abstract

Measles and canine distemper viruses were grown together in a Vero monkey kidney cell line. Each virus could be identified and individually titrated by using the color contrast produced by the reddish tetra-methyl rhodamine isothiocyanate-tagged antimeasles conjugate and the green fluorescein isothiocyanate-tagged antidistemper conjugate. Both blue light and green light were used for the excitation of the fluorochromes. Incident light was transmitted to the specimen by a vertical illuminator of the Ploem type.

MeSH Terms

Animals Cell Line Distemper Virus, Canine/growth & development,isolation & purification Dogs Fluorescent Dyes Haplorhini Horses Immune Sera Kidney Light Measles virus/growth & development,isolation & purification Methods Microscopy, Fluorescence Photomicrography Viral Vaccines Virus Cultivation

Chemicals

Fluorescent Dyes Immune Sera Viral Vaccines

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Gourlay J A

Pemberton J R

References (8)

8 references, click to expand

Development of a cell culture system susceptible to measle, canine distemper, and rinderpest viruses.
Arch Gesamte Virusforsch. 1967;22(3):364-80 PMID: 5600774
An immunofluorescence procedure for the detection of intracellular immunoglobulins.
Clin Exp Immunol. 1969 Apr;4(4):457-72 PMID: 4182405
Measles vaccine for protection of dogs against canine distemper.
J Am Vet Med Assoc. 1970 Jun 15;156(12):1743-6 PMID: 4912308
Early immunization against canine distemper and hepatitis, using combined vaccines.
J Am Vet Med Assoc. 1970 Jun 15;156(12):1755-8 PMID: 5463593
Propagation in tissue cultures of cytopathogenic agents from patients with measles.
Proc Soc Exp Biol Med. 1954 Jun;86(2):277-86 PMID: 13177653
Neutralisation of canine-distemper virus by serum of patients convalescent from measles.
Lancet. 1957 Aug 17;273(6990):344 PMID: 13464060
Propagation of canine distemper virus in tissue culture.
Proc Soc Exp Biol Med. 1959 Mar;100(3):551-4 PMID: 13634205
The relationships of the viruses of measles, canine distemper, and rinderpest.
Adv Virus Res. 1960;7:27-60 PMID: 13783209

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.22.3.459-462.1971

Published

1971-09-00

Pages

459-62

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC376333

Subset

External Links

PubMed source DOI: 10.1128/am.22.3.459-462.1971 PMC Full Text

Analysis Services

Simultaneous use of contrasting fluorochromes to separate measles and canine distemper viruses in a common system.

Contact

WeChat Official Account