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PMID: 5005302 Published · ppublish English Journal Article

Micro complement fixation assay for type-specific group A streptococcal antibody.

Infection and immunity ·Vol. 4 ·No. 4 ·1971-10-00 ·Pages 441-5

Wittner MK, Fox EN

Abstract

A micro complement fixation assay was devised to measure the type-specificity of anti-M antibody. Unabsorbed sera were from rabbits hyperimmunized with heat-killed streptococci and from humans with naturally occurring antibody or immunized with purified M protein vaccines. These sera fixed complement only in the presence of homologous M proteins (serotypes 1, 3, 6, 12, and 14). The complement fixation reaction paralleled the results obtained with the Lancefield bactericidal (opsonic) assay and did not exhibit the cross-reactions frequently seen with type-specific anti-M sera assayed by passive hemagglutination. All serological activity of the antisera resided in the fraction corresponding to 7S globulin eluted from a Sephadex G-200 gel filtration column. Because of the close correlation between micro complement fixation and the bactericidal assay for type-specific antibody, it is proposed that the micro complement fixation procedure, as we have described it, merits further evaluation as a substitute for the bactericidal test of immunity to group A streptococcal infection.

MeSH Terms
Animals Antibodies, Anti-Idiotypic/analysis Antibodies, Bacterial/analysis,isolation & purification Antigens, Bacterial/isolation & purification Bacterial Proteins/isolation & purification Bacterial Vaccines Complement Fixation Tests Evaluation Studies as Topic Hemagglutination Tests Humans Immunodiffusion Immunoglobulin G/analysis Immunoglobulin M/analysis Infant Rabbits/immunology Spectrophotometry, Ultraviolet Streptococcus/immunology
Chemicals
Antibodies, Anti-Idiotypic Antibodies, Bacterial Antigens, Bacterial Bacterial Proteins Bacterial Vaccines Immunoglobulin G Immunoglobulin M
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wittner M K
Fox E N
References (16)
16 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.4.4.441-445.1971
Published
1971-10-00
Pages
441-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC416328
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.4.4.441-445.1971 PMC Full Text View journal details
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