Separation of two types of adeno-associated virus particles containing complementary polynucleotide chains.

Journal of virology ·Vol. 9 ·No. 2 ·1972-02-00 ·Pages 394-6

Berns KI, Adler S

Abstract

An adeno-associated virus containing bromodeoxyuridine-substituted deoxyribonucleic acid has been fractionated by equilibrium centrifugation in CsCl into two classes of virions which contain complementary polynucleotide chains.

MeSH Terms

Adenoviridae/analysis Bromodeoxyuridine/metabolism Carcinoma Cell Line Centrifugation, Density Gradient DNA, Single-Stranded/analysis DNA, Viral/analysis Helper Viruses Humans Methods Mouth Neoplasms Nucleic Acid Denaturation Nucleic Acid Renaturation Phosphorus Isotopes Polynucleotides/analysis Tritium

Chemicals

DNA, Single-Stranded DNA, Viral Phosphorus Isotopes Polynucleotides Tritium Bromodeoxyuridine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Berns K I

Adler S

References (7)

7 references, click to expand

Nucleic acid from an adeno-associated virus: chemical and physical studies.
Proc Natl Acad Sci U S A. 1966 Jul;56(1):86-92 PMID: 5229859
The DNA of a minute virus of mice.
J Gen Virol. 1969 Jan;4(1):37-46 PMID: 4975639
Plus and minus single-stranded DNA separately encapsidated in adeno-associated satellite virions.
Science. 1969 Dec 5;166(3910):1280-2 PMID: 5350322
Evidence for a single-stranded adenovirus-associated virus genome: formation of a DNA density hybrid on release of viral DNA.
Proc Natl Acad Sci U S A. 1969 Nov;64(3):863-9 PMID: 5264145
Physicochemical characterization of adeno-associated satellite virus type 4 and its nucleic acid.
J Virol. 1967 Oct;1(5):980-7 PMID: 4987175
Evidence for a single-stranded adenovirus-associated virus genome: isolation and separation of complementary single strands.
J Virol. 1970 Jun;5(6):693-9 PMID: 5429749
Amino acid metabolism in mammalian cell cultures.
Science. 1959 Aug 21;130(3373):432-7 PMID: 13675766

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.9.2.394-396.1972

Published

1972-02-00

Pages

394-6

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356306

Subset

External Links

PubMed source DOI: 10.1128/JVI.9.2.394-396.1972 PMC Full Text View journal details

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Separation of two types of adeno-associated virus particles containing complementary polynucleotide chains.

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