Virus-associated nucleases: evidence for endonuclease and exonuclease activity in rabbitpox and vaccinia viruses.

Journal of virology ·Vol. 9 ·No. 3 ·1972-03-00 ·Pages 554-6

Aubertin AM, McAuslan BR

Abstract

The endonucleolytic action of a deoxyribonuclease activity in rabbitpox and vaccinia virus was established by change in sedimentation rate of denatured (3)H-lambda deoxyribonucleic acid substrate. The presence of two deoxyribonuclease activities in pox-virus is confirmed. Exo- and endonuclease activities are unmasked by treatment of purified virus with the detergent Nonidet P-40 and further enhanced by treatment of viral "cores" with trypsin.

MeSH Terms

Centrifugation, Density Gradient Chromatography, Gel DNA, Viral Deoxyribonucleases/metabolism Enzyme Activation Hydrogen-Ion Concentration Nucleic Acid Denaturation Poxviridae/enzymology Sucrose Surface-Active Agents Tritium Trypsin Vaccinia virus/enzymology

Chemicals

DNA, Viral Surface-Active Agents Tritium Sucrose Deoxyribonucleases Trypsin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Aubertin A M

McAuslan B R

References (6)

6 references, click to expand

The use of gel filtration to distinguish between endonucleolytic and exonucleolytic types of degradation.
Biochim Biophys Acta. 1966 Apr 18;119(1):198-200 PMID: 4289670
Two deoxyribonuclease activities within purified vaccinia virus.
Proc Natl Acad Sci U S A. 1969 Jul;63(3):820-7 PMID: 5259766
A DNA-depenent DNA polymerase and a DNA endonuclease in virions of Rous sarcoma virus.
Nature. 1970 Oct 31;228(5270):424-7 PMID: 4320562
Vaccinia virus structural polypeptide derived from a high-molecular-weight precursor: formation and integration into virus particles.
J Virol. 1970 Dec;6(6):717-26 PMID: 4250367
Adenovirus endonuclease: association with the penton of adenovirus type 2.
J Mol Biol. 1971 Aug 28;60(1):45-64 PMID: 5572103
A SIMPLE ASSAY FOR DNA ENDONUCLEASES.
Anal Biochem. 1965 Apr;11:133-7 PMID: 14328634

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.9.3.554-556.1972

Published

1972-03-00

Pages

554-6

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356332

Subset

External Links

PubMed source DOI: 10.1128/JVI.9.3.554-556.1972 PMC Full Text View journal details

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Virus-associated nucleases: evidence for endonuclease and exonuclease activity in rabbitpox and vaccinia viruses.

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