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PMID: 503860 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.

Nucleic acids research ·Vol. 7 ·No. 6 ·1979-11-24 ·Pages 1541-52

Kafatos FC, Jones CW, Efstratiadis A

Abstract

A dot hybridization method is presented for rapidly determining the relative concentrations of nucleic acids in a mixture, as well as the extent of sequence homology between related RNA or DNA species.

MeSH Terms

Base Sequence Chemical Phenomena Chemistry DNA DNA Restriction Enzymes DNA, Viral Methods Nucleic Acid Denaturation Nucleic Acid Hybridization Plasmids RNA RNA, Messenger

Chemicals

DNA, Viral RNA, Messenger RNA DNA DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kafatos F C

Jones C W

Efstratiadis A

References (8)

8 references, click to expand

Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.
J Mol Biol. 1962 Jul;5:109-18 PMID: 14470099
Specific excision of the inserted DNA segment from hybrid plasmids constructed by the poly(dA). poly (dT) method.
Biochim Biophys Acta. 1976 Dec 13;454(3):587-91 PMID: 999917
Amplification and characterization of a beta-globin gene synthesized in vitro.
Cell. 1976 Jun;8(2):163-82 PMID: 61066
End labeling of enzymatically decapped mRNA.
Nucleic Acids Res. 1977 Dec;4(12):4165-74 PMID: 202926
Silkmoth chorion proteins: sequence analysis of the products of a multigene family.
Proc Natl Acad Sci U S A. 1978 Jan;75(1):390-4 PMID: 272655
Vitellogenin in Xenopus laevis is encoded in a small family of genes.
Cell. 1979 Mar;16(3):535-49 PMID: 455441
Kinetic complexity of RNA molecules.
J Mol Biol. 1972 Jan 14;63(1):21-39 PMID: 4622766
A membrane-filter technique for the detection of complementary DNA.
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/7.6.1541

Published

1979-11-24

Pages

1541-52

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342326

Subset

External Links

PubMed source DOI: 10.1093/nar/7.6.1541 PMC Full Text View journal details

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Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.

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