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PMID: 503863 Published · ppublish English Comparative Study Journal Article

Correlation between the presence of tRNA His GUG and the erythropoietic function in foetal sheep liver.

Nucleic acids research ·Vol. 7 ·No. 6 ·1979-11-24 ·Pages 1635-48

Landin RM, Boisnard M, Petrissant G

Abstract

Histidyl-tRNAs from foetal and adult sheep liver were compared to their reticulocyte counterparts. The combination of various techniques revealed the existence of two histidyl-tRNA species in reticulocytes, one of which was not retained on acetylated DBAE-cellulose columns and was guanylatable. Three histidyl-tRNA isoacceptors were identified in foetal liver. Two of these species were not adsorbed on acetylated DBAE-cellulose but only one was found to be guanylatable. An identical chromatographic behaviour on RPC-5 columns was observed for guanylated histidyl-tRNAs from both origins. These results suggest the occurrence of a GUG anticodon in these guanine-accepting tRNAs. In foetal liver the amount of guanylatable histidyl-tRNA was estimated to be 7% of the total tRNA population. This observation is in agreement with the erythropoietic function of liver during the foetal life.

MeSH Terms
Amino Acyl-tRNA Synthetases/metabolism Animals Erythropoiesis Fetus Guanine Histidine Liver/metabolism Organ Specificity RNA, Transfer/metabolism Reticulocytes/metabolism Sheep Transferases/metabolism
Chemicals
Histidine Guanine RNA, Transfer Transferases Amino Acyl-tRNA Synthetases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Landin R M
Boisnard M
Petrissant G
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.6.1635
Published
1979-11-24
Pages
1635-48
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342333
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.6.1635 PMC Full Text View journal details
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