p-Toluenethiol as an initiator of autolysis in bakers' yeast.

Journal of bacteriology ·Vol. 109 ·No. 2 ·1972-02-00 ·Pages 949-51

Arnold WN

Abstract

Cysteine or dithiothreitol enhances the rate of autolysis in toluene-treated yeast. p-Toluenethiol alone is even more effective and is recommended for the isolation of beta-fructofuranosidase. This suggests a more general application of p-toluenethiol in the isolation of enzymes from yeast.

MeSH Terms

Autolysis Cell Wall/drug effects,enzymology Cresols/pharmacology Cysteine/pharmacology Dithiothreitol/pharmacology Hydrogen-Ion Concentration Methods Saccharomyces/drug effects,enzymology Sucrase/isolation & purification,metabolism Time Factors Toluene/pharmacology

Chemicals

Cresols Toluene Sucrase Cysteine Dithiothreitol

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Arnold W N

References (4)

4 references, click to expand

Studies on yeast beta-fructofuranosidase. XVI. Partial purification.
Enzymologia. 1965 Nov 6;29(3):306-14 PMID: 5884156
Purification of the internal invertase of yeast.
J Biol Chem. 1968 Apr 10;243(7):1567-72 PMID: 5647272
Heat inactivation kinetics of yeast beta-fructofuranosidase. A polydisperse system.
Biochim Biophys Acta. 1969 Apr 22;178(2):347-53 PMID: 5772408
ISOLATION AND PROPERTIES OF INTACT MITOCHONDRIA FROM SPHEROPLASTS OF YEAST.
J Bacteriol. 1964 Dec;88:1762-73 PMID: 14240967

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.109.2.949-951.1972

Published

1972-02-00

Pages

949-51

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285251

Subset

External Links

PubMed source DOI: 10.1128/jb.109.2.949-951.1972 PMC Full Text View journal details

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p-Toluenethiol as an initiator of autolysis in bakers' yeast.

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